Quercetin, Resveratrol and Grape Extracts Conjugated Carbon Nanoparticles Multidrug Delivery System for Sono-Photodynamic Breast Cancer Treatment | ||||
| Egyptian Journal of Chemistry | ||||
| Articles in Press, Accepted Manuscript, Available Online from 07 July 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2025.387331.11800 | ||||
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| Authors | ||||
Samir Ali Abd El-Kaream   1; Tarek M. Mohamed 2; Hassan Saleh Abdullatif Saleh 3; Abdalla M. Khedr 4
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| 1Applied Medical Chemistry Department, Medical Research Institute, Alexandria University, Alexandria-Egypt | ||||
| 2Chemistry Department, Affiliated Faculty of Science, Tanta University- Tanta, Egypt | ||||
| 3Chemistry Department, Affiliated Faculty of Science, Tanta University- Tanta, Egypt | ||||
| 4Chemistry Department, Faculty of Science, Tanta University- Tanta, Egypt | ||||
| Abstract | ||||
| Background: Cancer, a complex disease that has long presented significant therapeutic obstacles, necessitates innovative strategies capable of overcoming the shortcomings of traditional therapies, which frequently cause severe side effects and produce inadequate results. In the realm of medical necessity, sono-photodynamic therapy (SPDT) integrated with plant-based nanotechnology has surfaced as an appealing approach for cancer treatment. The sensitizer is the key component of SPDT, which has the ability to convert photo- and sono-energy into cytotoxic compounds when exposed to photo- and sono-irradiation. Sono-photosensitizers (SPSs) designs still have issues with oxygen dependence, penetration, targeting, and photon absorption. Due to the rapid advancements of material science, numerous SPSs that generate cytotoxic species with superior safety, noninvasiveness, and selectivity for the treatment of cancers have been created. Aim of the work: The current study intends to offer a cutting-edge approach to treating activated cancer by utilizing quercetin, resveratrol, and grape extracts conjugated carbon nanoparticles (QRG-CNP) for sono-photodynamic breast cancer (BrCa) in vitro and in vivo treatment. Materials and Methods: The current work was conducted on BrCa cells (MCF7) human cells in vitro and on Ehrlich ascites carcinoma-breast cancer-bearing (EAC-BrCa) Swiss albino mice. The treatment study protocol was initiated after the EAC-BrCa implantation, and it involved daily administration of QRG-CNP with or without ultrasound (US) and/or laser (IRL) exposure for three minutes over a two-week period. We utilized QRG-CNP as SPDT sensitizer (SPS), an IRL and US are our two sources of energy. Results: The results manifested that CNP could be employed as efficient QRG delivering system to the target BrCa cells. Furthermore, QRG-CNP is a potential SPS that can be highly successful in treating BrCa-MCF7 in vitro when combined with SPDT; (cell viability was declined, and cell death was induced; in a dose-dependent manner p<0.001*) and in vivo (EAC-BrCa-bearing mice; induction of antiproliferative apoptotic (Bax p<0.001*, p53 p<0.001*, TNFalpha p<0.001*, caspase 3,9 p<0.001*) genes, suppression of antiangiogenic (VEGF p<0.001*) and antiapoptotic (Bcl2 p<0.001*) genes, efficiently eliminating cancer cells, restoration of deteriorated parameter; enhancing the functions of the kidneys (urea, creatinine), liver (ALT, AST), conserving redox status homeostasis via regaining antioxidants balance (GPx p<0.001*, GSH p<0.001*, GR p<0.001*, GST p<0.001*, SOD p<0.001*, CAT p<0.001*, TAC p<0.001*), and lowering oxidative stress (MDA p<0.001*). This process can be connected to QRG sensitization upon the sono- and photo- chemical SPDT activation mechanism, and the non-activated QRG antioxidant potential. Conclusion: Our research findings indicate that QRG-CNP shows significant potential as an innovative, effective strategy of delivery for SPDT-activated breast cancer treatment. | ||||
| Keywords | ||||
| Targeted Therapy; Breast Cancer; Quercetin; Resveratrol; Grape; Carbon Nanoparticle; Sono-photodynamic | ||||
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