Detoxification of Hexavalent Chromium Using Biofilm Forming Paenochrobactrum pullorum Isolated from Tannery Wastewater Effluents | ||
| Al-Azhar Bulletin of Science | ||
| Volume 2023, Issue 1, January 2023 | ||
| DOI: 10.58675/2636-3305.1642 | ||
| Authors | ||
| Mohamad Elshabrawy1; Ahmed Labena2; Said Desouky1; Mohammed Barghoth1; Mohamed Salah Azab1 | ||
| 1Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt | ||
| 2Egyptian Petroleum Research Institute (EPRI), Nasr City, Cairo 11727, Egypt | ||
| Abstract | ||
| Bioremediation for wastewater using microbes that can detoxify the harmful effects of Cr-VI is concerned. Ninety-two isolates were collected from tannery effluents and screened based on their abilities to remediate hexavalent chromium using LB agar media supplemented by K2Cr2O7 as a source for Cr-VI. Out of the total isolates, 44 isolates can grow at 200 ppm of CR-VI, while 7, 6, 21, 11, and 3 isolates showed growth at concentrations of 400, 600, 800, 1000, and 1200 ppm of Cr-VI, respectively. Furthermore, determination of Cr-VI reduction efficiency for isolates was performed using 1,5-diphenylcarbazide (DPC) method, and the highest reduction efficiency (70.6%) was achieved by isolate 16R. This isolate was identified genetically based on the 16S rRNA gene with a higher similarity of 99.83% to Paenochrobactrum pullorum, and the genetic tree relationship was constructed. The optimization process was conducted to obtain the optimal reduction conditions, including contact time, pH, temperature, inoculum size, and Cr-VI concentration, using the One Factor at a Time (OFAT) method. The highest chromium reduction efficiency increased to 92.5% after optimization for the most potent isolate at pH 9, temperature 30-35°C, and inoculum size 3-4 ml after 5 days of incubation; biofilm formation was also represented. This study is the first to prove the chromium-reducing characteristic of this strain, along with its ability to form a biofilm, which strongly enhances the use of this strain in reduction and biological treatment processes in wastewater treatment contaminated with Cr-VI. | ||
| Keywords | ||
| Chromium-detoxification bacteria; Paenochrobactrum pullorum; Hexavalent chromium; Wastewater treatment; Biofilm-remediation | ||
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