Prevalence of Periodontal Pathogens in a Group of Egyptians with Emphasis on Correlation between Periodontal Stages and Molecular Bacterial Identification
Ramadan, A., El Kilani, N., Shaker, O., Ebrahim, A., Shoriebah, E. (2644). Prevalence of Periodontal Pathogens in a Group of Egyptians with Emphasis on Correlation between Periodontal Stages and Molecular Bacterial Identification. EKB Journal Management System, 2023(2), -. doi: 10.58675/2974-4164.1544
Amel Ramadan; Naglaa El Kilani; Olfat Shaker; Ahmed Ebrahim; Eatemad Shoriebah. "Prevalence of Periodontal Pathogens in a Group of Egyptians with Emphasis on Correlation between Periodontal Stages and Molecular Bacterial Identification". EKB Journal Management System, 2023, 2, 2644, -. doi: 10.58675/2974-4164.1544
Ramadan, A., El Kilani, N., Shaker, O., Ebrahim, A., Shoriebah, E. (2644). 'Prevalence of Periodontal Pathogens in a Group of Egyptians with Emphasis on Correlation between Periodontal Stages and Molecular Bacterial Identification', EKB Journal Management System, 2023(2), pp. -. doi: 10.58675/2974-4164.1544
Ramadan, A., El Kilani, N., Shaker, O., Ebrahim, A., Shoriebah, E. Prevalence of Periodontal Pathogens in a Group of Egyptians with Emphasis on Correlation between Periodontal Stages and Molecular Bacterial Identification. EKB Journal Management System, 2644; 2023(2): -. doi: 10.58675/2974-4164.1544

Prevalence of Periodontal Pathogens in a Group of Egyptians with Emphasis on Correlation between Periodontal Stages and Molecular Bacterial Identification

Al-Azhar Journal of Dentistry
Volume 2023, Issue 2, April 2023  XML
DOI: 10.58675/2974-4164.1544
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Authors
Amel Ramadan1; Naglaa El Kilani2; Olfat Shaker3; Ahmed Ebrahim4; Eatemad Shoriebah2
1Assistant lecturer in Oral Medicine, Periodontology, Oral Diagnosis and Dental Radiology Department. Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt.
2Professor of Oral Medicine, Periodontology, Diagnosis and Radiology Department, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt.
3Professor of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
4Professor of bioinformatics, Biology Department, school of science and Engineering, American University in Cairo, Egypt
Abstract
Purpose: This study was performed for identification and prevalence of periodontal microbiota in a group of Egyptians with a special emphasis on the correlation between different periodontal stages and molecular bacterial identification. Subjects and methods: 100 Subjects were divided equally into five groups with reference to new classification of periodontitis. Clinical parameters including probing pocket depth (PPD), clinical attachment level (CAL) gingival index (GI) and plaque index (PI) were measured. Samples of subgingival plaque were taken for microbiological analysis and bacterial DNA extraction. Bacterial identification was done using polymerase chain reaction (PCR) for 5 bacteria (Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Treponemadenticola (Td), Tannerellaforsythia (Tf), Fusobacterium nucleatum (Fn) in all samples for the study population. Results: The mean plaque index, gingival index, PD, and CAL were calculated, and the prevalence of P.g, T.d, T. f,A.a and F.n was also identified. Conclusion: for all kinds of bacteria the mean of real time increased by increasing the stage number. The difference between the healthy group and all stages was statistically significant. We can arrange the bacteria sequence as follows based on the overall mean of real time PCR for each type of bacteria: T.f,P.g, T.d,F.n,A.a. It was also clear that the relationship between PD and CAL and other bacterial microbiota was statistically significant.
Keywords
periodontal; diseases PCR; Egyptians
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