Experimental Study of Bone Defect Repair Using Centrifuged and Non-Centrifuged Bone Marrow Aspirate
El-Marzouky, H., Khalifa, F., Khalifa, G., Shamaa, A., Farid, M., ELsayed, S. (2640). Experimental Study of Bone Defect Repair Using Centrifuged and Non-Centrifuged Bone Marrow Aspirate. EKB Journal Management System, 6(1), -. doi: 10.21608/adjg.2019.28395
Hanaa H El-Marzouky; Fatma A Khalifa; Ghada A Khalifa; Ashraf A Shamaa; Mona H Farid; Shadia ELsayed. "Experimental Study of Bone Defect Repair Using Centrifuged and Non-Centrifuged Bone Marrow Aspirate". EKB Journal Management System, 6, 1, 2640, -. doi: 10.21608/adjg.2019.28395
El-Marzouky, H., Khalifa, F., Khalifa, G., Shamaa, A., Farid, M., ELsayed, S. (2640). 'Experimental Study of Bone Defect Repair Using Centrifuged and Non-Centrifuged Bone Marrow Aspirate', EKB Journal Management System, 6(1), pp. -. doi: 10.21608/adjg.2019.28395
El-Marzouky, H., Khalifa, F., Khalifa, G., Shamaa, A., Farid, M., ELsayed, S. Experimental Study of Bone Defect Repair Using Centrifuged and Non-Centrifuged Bone Marrow Aspirate. EKB Journal Management System, 2640; 6(1): -. doi: 10.21608/adjg.2019.28395

Experimental Study of Bone Defect Repair Using Centrifuged and Non-Centrifuged Bone Marrow Aspirate

Al-Azhar Journal of Dentistry
Volume 6, Issue 1, January 2019
DOI: 10.21608/adjg.2019.28395
Authors
Hanaa H El-Marzouky1; Fatma A Khalifa2; Ghada A Khalifa3; Ashraf A Shamaa4; Mona H Farid5; Shadia ELsayed6
1Dentist at Egyptian ministry of health,
2Associate Professor of oral and maxillofacial Surgery Faculty of Dental Medicine for Girls’ Al Azhar University,
3Professor Of oral and maxillofacial Surgery Faculty of Dental Medicine for Girls’ Al Azhar University,
4Professor of Surgery Anesthesiology and Radiology Faculty of Veterinary Medicine Cairo University,
5Professor of Oral Biology Faculty of Dental Medicine for Girls’ Al Azhar University,
6ِِAssistant professor of oral and maxillofacial surgery, faculty of Dental Medicine for Girls' Al Azhar University, cairo, Egypt.
Abstract
Purpose this study was conducted between 2015 and 2017 at the faculty of Veterinary Medicine Cairo University and aimed to histologically compare between the effect of processed and unprocessed bone marrow aspirate on the quantity and quality of the newly formed bone. Material and methods 72 Adult male white New Zealand rabbits were used in this study and were divided equally into 2 groups A and B. Bone defects were created at the medial upper extremity of right and left tibia of all rabbits down to bone using trephine bur measuring 4 mm diameter to 5 mm depth. The bone defects were treated with whole bone marrow loaded on gel foam in group A right tibias and using mononuclear cell layer obtained through centrifuging bone marrow loaded on gel foam in group B right tibias. Bone defects in the left tibias in all rabbits treated with gel foam only. Scarification was done 3,6,12 weeks postoperative. Histological evaluation was done using H and E and Masson’s trichrome stain. Results the results showed that group A right tibial defects had better healing results than group B right tibial defects all over the follow-up period, but the differences were not statically significant. Conclusion the study findings indicate that the centrifugal concentration techniques provide better therapeutic outcomes over the whole BMA, but it is statistically insignificant.
Keywords
bone; centrifuged; marrow; non-centrifuged; STEM
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