Efficacy of Chitosan as Final Irrigating Solution on TGF-β1 Release from Root Canal Dentin and its Effect on Dental Pulp Stem Cells Response | ||
| Al-Azhar Journal of Dentistry | ||
| Volume 8, Issue 2, April 2021, Pages 231-241 PDF (1020.14 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/adjg.2021.28468.1246 | ||
| Authors | ||
| Asmaa Abd El-Hady1; Wael Kamel2; Mona Farid3; Dina Sabry4 | ||
| 1Assistant Lecturer of Endodontics, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt. | ||
| 2Professor and Head of Endodontic Department, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt. | ||
| 3Professor of oral and dental biology, Former Dean of the Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt. | ||
| 4Professor of medical biochemistry and molecular biology, Faculty of Medicine, Cairo University, Cairo, Egypt. | ||
| Abstract | ||
| Purpose: To study the effect of Chitosan final irrigating solution compared with EDTA using different irrigation techniques, on TGF-β1 release from root canal dentin, using enzyme-linked immunosorbent assay (ELISA), and to evaluate the effect of released TGF-β1 on Dental Pulp Stem Cells. Materials and Methods: Human DPSCs were isolated from impacted third molars. Forty eight human root segments were prepared to a standardized truncated cone-shaped canal with an open apex of 1 mm diameter, the volume of prepared segments were determined using CBCT. Samples were randomized into two experimental groups (n=24) regarding the final irrigating solution; Group I: 1.5% NaOCl followed by 17% EDTA, Group II: 1.5% NaOCl followed by 1% Chitosan, then subdivided into subgroups A and B (n = 12) according to the final irrigation technique: A: Needle Irrigation, B: XP-Endo Finisher agitation. The released TGF-β1 were quantified using ELISA at 4 hours, 1 day, and 3 days following irrigation. DPSCs response to1.5% NaOCl, 17% EDTA, 1% Chitosan, and different concentrations of released TGF- β1 was assessed using MTT assay.Results: No statistically significant difference in the quantity of TGF-β1 released by 17% EDTA and 1% Chitosan following needle irrigation or XP Endo Finisher agitation. 1% Chitosan significantly increased DPSCs viability compared to17% EDTA (P < 0.05). TGF-β1 showed significant expansion of viable DPSCs in a positive correlation to its concentration (P <0.001). Conclusions: 1% Chitosan effectively released TGF-β1 from root canal dentin as17% EDTA. The released TGF-β1 promoted DPSCs proliferation at picogram levels in a concentration dependent manner. | ||
| Keywords | ||
| Chitosan; TGF-β1; Dental pulp stem cells | ||
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