The Effect of Incorporating Nanocalcium Phosphate Particles into Biodentine on Pulpal Tissue Response (In Vitro Study) | ||||
| Al-Azhar Journal of Dentistry | ||||
| Volume 2018, Issue 4, April 2018 | ||||
| DOI: 10.21608/adjg.2018.9529 | ||||
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| Authors | ||||
| Nawal Aidaros1; Maha Niazy2; Mohamed El-yassaky3 | ||||
| 1Assistant lecturer of Operative Dentistry, Faculty of Dentistry, Ahram Canadian University (ACU). | ||||
| 2Professor of Operative Dentistry, Faculty of Dental Medicine for Girl’s, AL-Azhar University. | ||||
| 3Professor of Operative Dentistry, and Dean of Faculty of Dental Medicine Sinai University. | ||||
| Abstract | ||||
| Objectives: This study was conducted to evaluate the effect of incorporating Nanocalcium phosphate (NCP) particles into Biodentine™ on pulpal tissue response as a direct pulp capping material (entire tooth culture model). Materials and methods: - A total of forty freshly human premolar teeth extracted for orthodontic reasons were used. After cavity preparation and mechanical pulp exposure, the teeth were divided into 2 main groups (20 teeth each) according to the capping material used; where group (M1) refers to pulps capped with Biodentine™, and group (M2) refers to pulps capped with Biodentine incorporated with NCP. Each group was further subdivided into two subgroups according to the culture period; where subgroup (P1) refers to teeth cultured for 14 days, and subgroup (P2) refers to teeth cultured for 28 days. At the end of each culture period, the teeth were histologically examined to assess the dentine bridge thickness of each group after 14 and 28 days. Moreover, elemental analysis of Calcium and Phosphorous ions in the dentine bridge was performed using SEM-EDX. Results: Direct pulp capping with Biodentine incorporated with NCP showed thicker dentine bridge when compared to Biodentine™ alone. There is a positive correlation between the weight % of Calcium and Phosphorous ions in the dentine bridge below both capping materials and the dentine bridge thickness. Conclusion: Biodentine incorporated with NCP could be a good candidate for pulp capping in the future | ||||
| Keywords | ||||
| Biodentine™; Calcium phosphate nanoparticles; entire tooth culture model | ||||
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