Tooth Repair by Treated Dentin Matrix Alone and Impregnated with Bone Marrow Mesenchymal Stem Cells in Rabbits | ||||
| Al-Azhar Journal of Dentistry | ||||
| Volume 2023, Issue 2, April 2023 | ||||
| DOI: 10.58675/2974-4164.1539 | ||||
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| Authors | ||||
| Dina Elbarky1; Heba Adawy2; Mena Fathy3 | ||||
| 1BDS, Faculty of Dental Medicine, Sinai University, Egypt | ||||
| 2Professor of Oral and Dental Biology, Head of Oral and Dental Biology Department, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt. | ||||
| 3Lecturer of Oral and Dental Biology, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt | ||||
| Abstract | ||||
| Purpose: This study aimed to see if treated dentin matrix paste (TDMP) alone and treated dentin matrix (TDM) powder combined with stem cells derived from bone marrow (BMMSCs) could induce the formation of calcific barrier after direct pulp capping procedures. Materials and methods: In this study 18 posterior teeth in 9 New Zealand white rabbits were involved and simple randomization design was used. All of the experimental teeth were subjected to direct pulp capping procedures. Eighteen teeth were divided into 3 groups, each one consisted of 6 teeth. In group I, calcium hydroxide (CH) was used, while TDMP and TDM powder impregnated with BMMSCs were used in group II and group III respectively. Modified glass ionomer was used as a sealing restoration. After four weeks, animals were euthanized and experimental teeth were demineralized and processed for histological examination to see if a hard tissue barrier was formed. Results: TDMP presented the best results where a continuous reparative dentin bridge with homogenous tubular structure was formed. On the other hand, CH stimulated the formation of non-continuous irregular hard tissue masses while seeding BMMSCs on TDM powder resulted in defect closure with a bone like material and dentin like islands were formed inside the adjacent pulp. Conclusion: pulp capping with TDMP without adding stem cells from other sources like bone marrow is more effective in producing a high quality dentin bridge through its effect in stimulating the resident cells inside the pulp. | ||||
| Keywords | ||||
| Pulp capping; Calcium hydroxide; dentin matrix; stem cells | ||||
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