Molecular Characterization of ESBLs and Carbapenemases in Pseudomonas aeruginosa Isolated from Different Food Products in Egypt | ||||
| Egyptian Journal of Veterinary Sciences | ||||
| Articles in Press, Corrected Proof, Available Online from 10 July 2025 PDF (921.28 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejvs.2025.365115.2677 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Doha Naeim  1; Amgad A. Moawad 2; Ashraf M. Ahmed1
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| 1bacteriology, immunology, and mycology, veterinary medicine, KFS university, KFS, Egypt | ||||
| 2Bacteriology, Mycology and Immunology Department, Faculty of Veterinary medicine, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt. | ||||
| Abstract | ||||
| This research was undertaken to estimate the prevalence of Extended spectrum Beta-lactamases (ESBLs) and AmpC beta-lactamases (AmpC), and blaNDM in P. aeruginosa from various food products in Egypt. A number of 300 food samples were randomly collected from different markets. We used standard biochemical and bacteriological methods for isolating and identifying all of the samples. Out of these 300 samples, 240 (80%) isolates were identified as P. aeruginosa. Out of 240 isolates, 200 (80%) demonstrated resistance phenotypes to two or more antimicrobial agents. Overall, the complete resistance (100%) was observed against cefoxitin, ampicillin (90.8%), cefoxitin (88.3%), while susceptibility of isolates was observed against aztreonam and colistin. The PCR- and DNA-sequencing screening recognized many narrow- and ESBL and carbapenemase -encoding genes in 16 (6.7%) bacterial isolates.The BlaCMY-2 β-lactamase encoding gene was identified in 2 (0.8%) isolates. The blaNDM-1 was identified as carbapenemase-encoding gene, which identified in 16 (6.7%) isolates. The elevated incidence of β-lactamase resistant genes in different foodstuffs in Egypt constitutes a serious public health threat with the possibility of transfer of these strains to people. | ||||
| Keywords | ||||
| ESBLs; blaNDM; Food; PCR; Pseudomonas aeruginosa | ||||
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