Genetic fingerprinting relationships between Escherichia coli and Staphylococcus aureus isolated from different sources | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 10 July 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2025.392517.2870 | ||||
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| Authors | ||||
Nagham Saadi Mohammed   1; Halah Al- Haideri 2; Mustafa Riyadh Abdullah 1; Tabarek mundher noaman 1
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| 1Department of pharmacology and toxicology/ College of pharmacy / Mustansiriyah University/ Baghdad, Iraq | ||||
| 2Department of Biology/ College of Science for Women/ University of Baghdad/ Baghdad, Iraq | ||||
| Abstract | ||||
| Background: Escherichia coli are generally harmless bacteria residing in the intestines of humans and animals. However, consuming food or water contaminated with certain strains of E. coli can lead to mild to severe gastrointestinal illness. Staphylococcus aureus is considered among the most opportunistic pathogens, responsible for causing infections in both immunocompetent and immunocompromised individuals. They are primarily carried in the nasal cavity, but can also be found on the skin. Objectives: The purpose of this study was to identify the clonal structures and genetic heterogeneity of Escherichia coli and Staphylococcus aureus through ERIC-PCR in order to enhance epidemiological investigation data to plan and execute appropriate management of the infections. Methods: 120 samples were collected from different sources, and a total of 112 isolates of E. coli and S. aureus were identified by traditional biochemical methods. The genomic DNA of all isolates was extracted and subjected to Enterobacterial Repetitive Intergenic Consensus –Polymerase chain Reaction (ERIC-PCR). The PCR products were electrophoresed and visualized by a UV-trans illuminator. The banding patterns were analyzed using UPGAMA software. Results: The results showed that the fingerprinting patterns of E. coli include 50 to 1500 bands, whereas, 100 to 1500 bands were included in S. aureus. The most predominant band was 1000 bp in E. coli and S. aureus. On the basis of the dendrogram of ERIC-PCR fingerprinting, there were 8 clusters of E. coli and S. aureus, while ER6 and ER8 had 18 isolates that had identical genotypes in E. coli. Thus, the identification of the DNA profile to molecular typing of E. coli and S. aureus isolates isolated from various sources using ERIC-PCR might be an effective too. Conclusions: ERIC-PCR is a crucial tool for epidemiological investigations of pathogens like E. coli and S. aureus, requiring standardization and international databases for global comparisons and investigations. | ||||
| Keywords | ||||
| Staphylococcus aureus; Escherichia coli; Molecular typing; Genotype | ||||
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