The Antimicrobial Efficacy of Modified Salt Solutions Against E. faecalis Biofilms: An Ex-Vivo Study
Heidar, S., Fathy, I., Elsherief, S. (2025). The Antimicrobial Efficacy of Modified Salt Solutions Against E. faecalis Biofilms: An Ex-Vivo Study. EKB Journal Management System, 71(3), 2725-2733. doi: 10.21608/edj.2025.382689.3467
Sherif Heidar; Iman Ahmed Fathy; Samia Mohamed Elsherief. "The Antimicrobial Efficacy of Modified Salt Solutions Against E. faecalis Biofilms: An Ex-Vivo Study". EKB Journal Management System, 71, 3, 2025, 2725-2733. doi: 10.21608/edj.2025.382689.3467
Heidar, S., Fathy, I., Elsherief, S. (2025). 'The Antimicrobial Efficacy of Modified Salt Solutions Against E. faecalis Biofilms: An Ex-Vivo Study', EKB Journal Management System, 71(3), pp. 2725-2733. doi: 10.21608/edj.2025.382689.3467
Heidar, S., Fathy, I., Elsherief, S. The Antimicrobial Efficacy of Modified Salt Solutions Against E. faecalis Biofilms: An Ex-Vivo Study. EKB Journal Management System, 2025; 71(3): 2725-2733. doi: 10.21608/edj.2025.382689.3467

The Antimicrobial Efficacy of Modified Salt Solutions Against E. faecalis Biofilms: An Ex-Vivo Study

Egyptian Dental Journal
Volume 71, Issue 3 - Serial Number 5, July 2025, Page 2725-2733  XML PDF (1.6 MB)
Document Type: Original Article
DOI: 10.21608/edj.2025.382689.3467
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Authors
Sherif Heidar email orcid 1; Iman Ahmed Fathyorcid 2; Samia Mohamed Elsherieforcid 3
1Lecturer, Endodontics Department, Faculty of Dentistry, Suez University.
2Associate Professor, Oral Biology Department (Stem Cells Research Unit), Faculty of Dentistry, Ain Shams University
3Professor, Endodontics Department, Faculty of Dentistry, Umm Al-Qura University; Cairo University.
Abstract
Objectives: The aim of this study was to investigate the disinfecting effect of a modified salt solution (MSS) on Enterococcus faecalis biofilms in ex-vivo model.
Methods: Eighteen human dentine sections were standardized, inoculated with E. faecalis and incubated for three weeks to ensure bacterial biofilm maturation in the root canal space. Then samples will be allocated according to irrigation into 3 groups (6 dentine sections each): GI (Control) was irrigated with distilled water, GII irrigated with modified salt solution (MSS) and GIII with sodium hypochlorite (NaOCl) irrigation. Afterwards dentine sections were stained with 1:1 mixture of Syto 9/propidium iodide for LIVE/DEAD staining of E. faecalis, and were analyzed under conofocal laser scanning microscopy (CLSM). Comparisons between groups were done by One Way ANOVA test followed by Tukey’s post hoc test for multiple comparisons.
Results: The results showed that both irrigants NaOCL and MSS dramatically increased E. faecalis death compared to the control. There was no statistically significant difference between MSS and NaOCl in dead E. faecalis detected in CLSM images.
Conclusion: MSS is potent antimicrobial against E. faecalis biofilm in dentine, with efficacy comparable to NaOCl.
Keywords
Sodium hypochlorite (NaOCl) irrigation; modified salt solution; conofocal laser scanning microscopy; E. faecalis biofilm
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