Isolation and identification of strawberry crown rot pathogen
Hafez, M., Essa, T., EL-Habbaa, G., Elsisi, A., Ahmed, G. (2025). Isolation and identification of strawberry crown rot pathogen. EKB Journal Management System, 10(3), 13-23. doi: 10.21608/bjas.2025.363123.1597
Mohamed Ahmed Hafez; Tarek Essa; Gehad A EL-Habbaa; Ahmed A Elsisi; Gamal Ahmed. "Isolation and identification of strawberry crown rot pathogen". EKB Journal Management System, 10, 3, 2025, 13-23. doi: 10.21608/bjas.2025.363123.1597
Hafez, M., Essa, T., EL-Habbaa, G., Elsisi, A., Ahmed, G. (2025). 'Isolation and identification of strawberry crown rot pathogen', EKB Journal Management System, 10(3), pp. 13-23. doi: 10.21608/bjas.2025.363123.1597
Hafez, M., Essa, T., EL-Habbaa, G., Elsisi, A., Ahmed, G. Isolation and identification of strawberry crown rot pathogen. EKB Journal Management System, 2025; 10(3): 13-23. doi: 10.21608/bjas.2025.363123.1597

Isolation and identification of strawberry crown rot pathogen

Benha Journal of Applied Sciences
Volume 10, Issue 3, March 2025, Page 13-23  XML PDF (615.61 K)
Document Type: Original Research Papers
DOI: 10.21608/bjas.2025.363123.1597
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Authors
Mohamed Ahmed Hafez email 1; Tarek Essa2; Gehad A EL-Habbaa1; Ahmed A Elsisi1; Gamal Ahmed1
1Department of Plant Pathology, Faculty of Agriculture, Benha University
2Junior Researcher, Plant pathology, Vegetable Diseases Department, Plant Pathology Institute, Agricultural Research Center, Giza
Abstract
Strawberry is a high-value crop for economy and nutrition. This study was conducted to identify of strawberry crown rot pathogen disease. Five fungal genera were isolated, purified and identified according to the phenotypic criteria studied: Pestalotiopsis spp., F. solani, F. oxysporum, Rhizoctonia solani, and Macrophomina phaseolina. Pestalotiopsis spp. fungus were the most common accounting for 42.3% of the total isolates. Moreover, F. oxysporum was isolated with a frequency of 23.7%, and F. solani with 14.8%. The pathogen isolated from crown root diseased strawberry plants was morphologically similar to the genus Neopestalotiopsis. Phylogenetic tree based on ITS sequences of rDNA of the fungal sample isolated in the present study (Pestalotiopsis microspora strain AUMC16335, arrowed) aligned with closely related strains accessed from the GenBank. This strain showed 99.81% identity and 100% coverage with several strains of the same species. A close relationship was also observed between the current strain sequences and some Pestalotiopsis haikouensis strains. Pestalotiopsis microspora strain (AUMC16336, arrowed) aligned with closely related strains accessed from the GenBank. This strain showed 99.81% identity and 100% coverage with several strains of the same species. Five strawberry varieties were evaluated for resistance to Pestalotiopsis. Both Sensation and Fortuna cultivars showed a significant increase in the disease incidence rate (DI), which they recorded at 93.33%. The Florida Beauty variety recorded an infection rate of 87.67%. The Winter Star and Festival varieties also recorded the lowest infection rate, with 80.00% and 73.33%.
Keywords
Strawberry; crown rot; Pestalotiopsis; GenBank; Neopestalotiopsis
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