Insecticidal Potency and Biochemical Activity of Beauveria bassiana and Cymbopogon schoenanthus Extract on Sitophilus zeamais (Coleoptera: Curculionidae)

Aamer, Neama A.; Gaber, Mahmoud A.; El-Moaty, Zeinab A.; Aamer, Helmy A.

doi:10.21608/alexja.2025.400193.1152

	Insecticidal Potency and Biochemical Activity of Beauveria bassiana and Cymbopogon schoenanthus Extract on Sitophilus zeamais (Coleoptera: Curculionidae)
Alexandria Journal of Agricultural Sciences
Articles in Press, Corrected Proof, Available Online from 13 July 2025 PDF (770.37 K)
Document Type: Original Article
DOI: 10.21608/alexja.2025.400193.1152
View on SCiNiTO
Authors
Neama A. Aamer¹; Mahmoud A. Gaber ²; Zeinab A. El-Moaty³; Helmy A. Aamer⁴
¹Department of Applied Entomology and Zoology, Faculty of Agriculture (El-Shatby), Alexandria University 21545, Egypt
²Department of Plant Pathology, Faculty of Agriculture (El-Shatby), Alexandria University 21545, Egypt
³Department of Zoology, Faculty of Science, Alexandria University, Moharam Bey, Alexandria 21511, Egypt
⁴Department of Pesticide Chemistry and Technology, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria 21545, Egypt
Abstract
Among the important postharvest challenges that pest grain infestation causes worldwide, one of the most destructive primary pests of cereal grains is the Sitophilus zeamais. The current study investigates and compares the biocontrol potential of the entomopathogenic fungus Beauveria bassiana and the ethyl acetate extract of Cymbopogon schoenanthus against S. zeamais in terms of evaluating insecticidal efficiency and biochemical effects on oxidative stress biomarkers. C. schoenanthus extracts yielded nine major bioactive compounds by GC-MS analysis, the predominant ones being α-eudesmol (43.9%), elemol (15.66%), and cryptomeridiol (13.38%). Bioassays indicated concentration-dependent mortality for both treatments: B. bassiana yielded 80.0% mortality at 14 days (LC₅₀: 1.08×10⁶ spores/mL), whereas C. schoenanthus extract yielded 73.0% (LC₅₀: 29.42 mg/mL). Both treatments reduced F₁ progeny production significantly by 81.5% (B. bassiana) and 70.6% (C. schoenanthus), where losses in weight of grains were only reduced to 2.4% and 5.2%, respectively, compared to the 30% loss observed in controls. Biochemical evaluation indicated different responses to oxidative stress. C. schoenanthus extract caused chronic but manageable oxidative stress with the sustained rise of the antioxidant enzymes (catalase: 1.53-fold, superoxide dismutase: 1.28-fold, glutathione S-transferase: 1.34-fold), and mild lipid peroxidation by 44.1% MDA increase. B. bassiana exhibited a biphasic pattern: initial enzyme upregulation followed by the total collapse of the antioxidant system at day five (20-22% below control levels), accompanying severe oxidative damage (71.2% MDA increase), synchronizing with increased mortality. Both fungal and botanical biocontrol agents are promising eco-friendly alternatives to synthetic pesticides for stored grain pest management, as this study's findings show
Keywords
Beauveria bassiana; Cymbopogon schoenanthus; Sitophilus zeamais; Biocontrol; Oxidative stress; Sesquiterpenes; stored grain; Antioxidant enzymes


Statistics Article View: 112 PDF Download: 41