Exploring IL-37 Genetic Variants and Circulating IL-37 Levels in Hepatitis B and C Infections: Insights from Hardy-Weinberg Equilibrium Analysis | ||||
| Egyptian Journal of Medical Microbiology | ||||
| Article 46, Volume 35, Issue 1, January 2026 | ||||
| Document Type: New and original researches in the field of Microbiology. | ||||
| DOI: 10.21608/ejmm.2025.400508.1756 | ||||
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| Authors | ||||
Sally Hassan1; Rana T. Mohsen  2
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| 1Department of Biology, College of Science, University of Al-Anbar, Iraq | ||||
| 2Department of Biotechnology, College of Science, University of Anbar, Al-Anbar, Iraq | ||||
| Abstract | ||||
| Background: Emerging evidence indicates that hereditary polymorphisms within The IL-37 gene are believed to influence an individual's vulnerability to various infectious and inflammatory disorders by modulating immune responses, including viral hepatitis. Objective: The study evaluated the distribution of IL-37 genotypes within the study population, with particular attention to their compliance with Hardy-Weinberg equilibrium (HWE), in order to confirm the genetic stability and representativeness of the sample Methodology :Total of 190 participants were included, comprising patients, During the period spanning September to December 2022, investigators carried out a study in Al-Anbar Governorate, enrolling 90 participants with chronic hepatitis and 100 healthy . Genomic DNA was isolated and genotyped for four IL-37 SNPs: rs2708967, rs2708971, rs3811047, and rs2466449, utilizing sequence-specific PCR techniques. Circulating IL-37 levels by (ELISA). Genotype frequencies were examined for HWE conformity and compared across groups. Statistical analyses was performed utilizing suitable parametric and non-parametric approaches to ensure accurate evaluation of the data. Results: the SNPs rs3811047 and rs2466449 exhibited statistically significant correlations (p < 0.05), These variants may influence individual susceptibility or resistance to viral infection. Certain genotypes deviated from Hardy-Weinberg equilibrium among patient groups, indicating possible selective disease associations. Furthermore, IL-37 serum levels were markedly high in infected individuals associated to controls, with some genotypes showing a significant association with increased protein expression. Conclusion: The observed upregulation of IL-37 in patients further supports its role in the host’s immunological response to chronic viral hepatitis. IL-37 may therefore serve as a promising genetic and immunological biomarker. | ||||
| Keywords | ||||
| IL-37; HBV; HCV; HWE | ||||
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