Antibacterial and antiproliferative activities of Streptomyces sp. strain MFB27 | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 14 July 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.384984.2794 | ||
| Authors | ||
| Zineb Azoubi* 1; Assia MOUSLIM1; Saad MENGGAD2; Nadia ZOUGAGH3; Ayoub ELABBASSI3; Nourddine HABTI4; Mohammed MENGGAD3 | ||
| 1department of Biology, Faculty of Sciences Ben M’sick, Hassan II University of Casablanca, Morocco | ||
| 2MRH Research Center, Department of Medicine, University of Montréal, Canada | ||
| 3depratment of Biology, Faculty of Sciences Ben M’sick, Hassan II University of Casablanca, Morocco | ||
| 4Faculty of medicine and pharmacy, Hassan II University of Casablanca, Morocco | ||
| Abstract | ||
| Background: Novel and effective bioactive compounds are needed as the always emerging incidence of drug resistance and with multi-resistance in some cases. The Streptomyces sp. MFB27 strain, was previously isolated from soil sample in Rabat, Morocco. Objective: This study aimed to investigate the antibacterial and antiproliferative potential of the strain MFB27. Methods: Bioactive metabolites were extracted from mycelium and culture supernatant by using ethanol and chloroform yielding ethanol chloroform and aqueous extracts. The extracts were assessed for their antibacterial activity through disk diffusion technique against clinical test strains. Their antiproliferative effect toward P3 mice myeloid and U2OS human osteosarcoma cell lines was performed by MTT assay. Results: The results showed that all extracts presented significant activity on Staphylococcus aureus and Micrococcus luteus. Moreover, these extracts inhibited proliferation of the two different cancer cell lines used. FACs analysis on the treated U2OS human osteosarcoma cell line exhibited cell cycle phase arrests at subG1, S and G2/M. Morphological features of extract treated U2OS cells showed similar characteristic of apoptosis. Conclusion: This study demonstrated that these extracts could be exploited to efficiently treat cancer and bacterial infections. | ||
| Keywords | ||
| antibacterial; antiproliferative; cancer cells; cycle phase arrest; Streptomyces sp | ||
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