Antibacterial and antiproliferative activities of Streptomyces sp. strain MFB27

Azoubi, Zineb; MOUSLIM, Assia; MENGGAD, Saad; ZOUGAGH, Nadia; ELABBASSI, Ayoub; HABTI, Nourddine; MENGGAD, Mohammed

doi:10.21608/mid.2025.384984.2794

	Antibacterial and antiproliferative activities of Streptomyces sp. strain MFB27
Microbes and Infectious Diseases
Articles in Press, Accepted Manuscript, Available Online from 14 July 2025
Document Type: Original Article
DOI: 10.21608/mid.2025.384984.2794
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Authors
Zineb Azoubi ¹; Assia MOUSLIM¹; Saad MENGGAD²; Nadia ZOUGAGH³; Ayoub ELABBASSI³; Nourddine HABTI⁴; Mohammed MENGGAD³
¹department of Biology, Faculty of Sciences Ben M’sick, Hassan II University of Casablanca, Morocco
²MRH Research Center, Department of Medicine, University of Montréal, Canada
³depratment of Biology, Faculty of Sciences Ben M’sick, Hassan II University of Casablanca, Morocco
⁴Faculty of medicine and pharmacy, Hassan II University of Casablanca, Morocco
Abstract
Background: Novel and effective bioactive compounds are needed as the always emerging incidence of drug resistance and with multi-resistance in some cases. The Streptomyces sp. MFB27 strain, was previously isolated from soil sample in Rabat, Morocco. Objective: This study aimed to investigate the antibacterial and antiproliferative potential of the strain MFB27. Methods: Bioactive metabolites were extracted from mycelium and culture supernatant by using ethanol and chloroform yielding ethanol chloroform and aqueous extracts. The extracts were assessed for their antibacterial activity through disk diffusion technique against clinical test strains. Their antiproliferative effect toward P3 mice myeloid and U2OS human osteosarcoma cell lines was performed by MTT assay. Results: The results showed that all extracts presented significant activity on Staphylococcus aureus and Micrococcus luteus. Moreover, these extracts inhibited proliferation of the two different cancer cell lines used. FACs analysis on the treated U2OS human osteosarcoma cell line exhibited cell cycle phase arrests at subG1, S and G2/M. Morphological features of extract treated U2OS cells showed similar characteristic of apoptosis. Conclusion: This study demonstrated that these extracts could be exploited to efficiently treat cancer and bacterial infections.
Keywords
antibacterial; antiproliferative; cancer cells; cycle phase arrest; Streptomyces sp


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