Combining Gene Expression and Candidate Gene Approach for Improvement of Growth Traits in Dromedary Camels | ||
| Egyptian Journal of Chemistry | ||
| Volume 69, Issue 1, January 2026, Pages 1-13 PDF (1.43 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejchem.2025.386502.11790 | ||
| Authors | ||
| Ahmed Moussa Amer1; Amal Abdel-Kreem Othman1; Mohamed Ibrahim Shehab El-Din2; mohammed atef kamal El den3; Ahmed Ibrahim Ateya4; Esteftah Mohamed El-Komy* 5; Shimaa A. Sakr6 | ||
| 1Department of Camel Research, Animal Production Research Institute, Agricultural Research Center, Dokki, Giza, Egypt. | ||
| 2Animal Production Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt | ||
| 3Department of Animal Productions, Faculty of Agriculture, Al-Azhar University, Assiut, Egypt. | ||
| 4Animal Wealth Development Department... Faculty of. Veterinary Medicine... Mansoura University | ||
| 5Department of Animal Production, National Research Centre, 12622, Dokki, Giza, Egypt | ||
| 6Animal Wealth Development Department, Faculty of. Veterinary Medicine, Mansoura University | ||
| Abstract | ||
| The present research aimed to ascertain the relationship between gene expression and single nucleotide polymorphisms (SNPs) of the genes encoding glutamate decarboxylase (GAD1), fatty acid binding protein 4 (FABP4), transmembrane protein 95 (TMEM95), nucleobindin 2 (NUCB2), ATP binding cassette sub-family G member 2 (ABCG2), signal transducer and activator of transcription 5A (STAT5A), and uncoupling protein 1 (UCP1) may be related to growth traits in dromedary camels. Farm records documented the growth characteristics of 340 male dromedary camels. Following genotyping analysis, a total of 72 male dromedary camels were selected based on the availability of their phenotype records and similar conditions. Blood samples were obtained from the 72 selected individuals to perform the RNA extraction procedure. PCR-DNA sequencing of UCP1, TMEM95, NUCB2, FABP4, ABCG2, STAT5A, and GAD1 revealed SNP variations in the amplified DNA regions. These SNPs were used to classify the camels into distinct genotype groups for further analysis. A significant correlation (P < 0.01) was observed between growth traits and the detected SNPs, accompanied by notable differences in the expression profiles (UCP1, TMEM95, NUCB2, FABP4, ABCG2, STAT5A, and GAD1) among dromedary camels, which indicates their potential as surrogates for growth rates. Furthermore, dromedary camels with high growth rates may benefit from marker-assisted selection (MAS) using the identified SNPs and the studied gene expression profiles. The results may support future MAS in camels, pending further validation. | ||
| Keywords | ||
| Candidate gene; Dromedary camels; Gene expression; Growth traits | ||
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