Molecular Detection and Characterization of Staphylococcus aureus and Escherichia coli Isolated From Food of Animal Origin | ||||
| Egyptian Journal of Veterinary Sciences | ||||
| Articles in Press, Accepted Manuscript, Available Online from 15 July 2025 PDF (916.62 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejvs.2025.381254.2843 | ||||
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| Authors | ||||
Nancy Hesham Abd-Elmgeed1; Mayada Gwida2; Amro Mohamed2; Adel Helmy Elgohary  3
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| 1Department of Hygiene and Zoonoses Mansoura University | ||||
| 2Department of Hygiene and Zoonoses, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt | ||||
| 3Hygiene and Zoonoses Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura City, Egypt | ||||
| Abstract | ||||
| Zoonotic diseases are a major global threat to public health and animal welfare. The development of drug resistance among these zoonotic pathogens has been associated with intensive and uncontrolled usage of antibiotics in food-producing animals. The current study was carried out on 210 different types of foods of animal origin including beef, chicken raw meat, raw milk and karish cheese as well as hand swabs from food handlers. The aim of the present work was to characterize Staphylococcus aureus (S. aureus), and Escherichia coli (E. coli) isolated from food of animal origin. In the current study the overall prevalence of S. aureus and E. coli from food of animal origin were 33(15%) and 24 (11.4%), respectively. Also, this study identified various serotypes of E. coli, including Enterotoxigenic Escherichia coli (ETEC) (25%), Enteropathogenic Escherichia coli (EPEC) (33%), Enterohemorrhagic (EHEC) (37%), and Entero-invasive Escherichia coli (EIEC) (4.1%), with some strains harboring virulent genes such as shiga toxin 1 (stx1) (29%), shiga toxin 2 (stx2) (29%), hemolysin toxin (hylA) (20.8%), and intimin (eaeA) (16%). The identified E. coli strains showed 100% resistance to vancomycin while the lower resistance rate was reported to colistin, with an average MAR index of 0.471. Staphylococcus aureus strains exhibited 100% resistance to kanamycin meanwhile the lower resistance rate was to daptomycin, with an average MAR index of 0.439. MDR strains of E. coli and S. aureus isolated from food samples and food handlers are creating risk hazards and threats to consumers that necessitate the continuous surveillance and implementation of control regimes to reduce the inappropriate use of antimicrobial to mitigate the spread of various resistance genes as well as pathogenic strains to meat consumers. | ||||
| Keywords | ||||
| Genetic charcterization; foodborne pathogens; Animal origin | ||||
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