Characterization, antioxidant, antimicrobial and antitumor activities of silver nanoparticles synthesized by fungi | ||||
| Bulletin of Pharmaceutical Sciences Assiut University | ||||
| Articles in Press, Accepted Manuscript, Available Online from 15 July 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bfsa.2025.384858.2559 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Mohammed Hamdy Abdel-hafeez  1; Ahmed Mohammed Moharram1; Abdel Naser Ahmed Zohri2
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| 1Assiut University Moubasher Mycological Centre (AUMMC), Assiut University, Assiut, Egypt | ||||
| 2Department of Botany and Microbiology, Faculty of Science, Assiut University, Assiut 71511, Egypt | ||||
| Abstract | ||||
| Abstract: Because metal nanoparticles are used in many different fields, there has a lot of interest in their green synthesis. Fifteen isolates of fungi isolated from Egyptian popcorn and rice has been evaluated for their ability to synthesis AgNPs. The antioxidant, antimicrobial and antitumor potentiality of AgNPs were examined. Out of 15 tested fungal species, Trichoderma harzianum AUMMC 17008 was the best that reduce silver salt into AgNPs. The formation of AgNPs was confirmed by UV-Vis., TEM showed distinct shape and size of nanoparticles. X-ray diffraction studies the crystalline structure of the AgNPs. The antioxidant activity of AgNPs in DPPH assay shows the highest inhibition rise with increasing concentration, AgNPs showed antibacterial activity against different bacterial species via the agar well diffusion assay. The inhibition zones varied from 11 to 24 mm against 5 human pathogenic bacterial species. Also, AgNPs showed antifungal behavior against aflatoxigenic isolate of Aspergillus flavus. Concentrations 0.25 mM and 0.5 mM of AgNPs completely inhibit the growth and toxin formation of Aspergillus flavus, while the other concentration of AgNPs didn’t exhibit any effect on both the growth and toxin formation. For the effect of AgNPs on antitumor indicate that AgNPs were able to decrease the cell viability of MDA-MB231 cells in a dose-dependent manner after the cells were treated with different concentrations of AgNPs (31.25–1000 𝜇g/mL) for 24 hours. AgNPs were found to be cytotoxic to the cells at concentrations of 31.25 𝜇g/mL and above after 24 hours of treatment | ||||
| Keywords | ||||
| Filamentous fungi; biosynthesis, nanomedicine; silver nanoparticles; antimicrobial activity | ||||
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