The Possible Ameliorating Effect of Lactoferrin as adjuvant to Ceftriaxone on Induced Intestinal Sepsis in adult male albino rats (Histological and Immunohistochemical Study)
Refaat, G., Zaki, M., Shenouda, H., Bayoumi, H. (2025). The Possible Ameliorating Effect of Lactoferrin as adjuvant to Ceftriaxone on Induced Intestinal Sepsis in adult male albino rats (Histological and Immunohistochemical Study). EKB Journal Management System, 42(8), 33-50. doi: 10.21608/bmfj.2025.384806.2409
Gehad Sherif Refaat; Mohamed Magdi Zaki; Helpies Delwar Shenouda; Heba Elsayed Bayoumi. "The Possible Ameliorating Effect of Lactoferrin as adjuvant to Ceftriaxone on Induced Intestinal Sepsis in adult male albino rats (Histological and Immunohistochemical Study)". EKB Journal Management System, 42, 8, 2025, 33-50. doi: 10.21608/bmfj.2025.384806.2409
Refaat, G., Zaki, M., Shenouda, H., Bayoumi, H. (2025). 'The Possible Ameliorating Effect of Lactoferrin as adjuvant to Ceftriaxone on Induced Intestinal Sepsis in adult male albino rats (Histological and Immunohistochemical Study)', EKB Journal Management System, 42(8), pp. 33-50. doi: 10.21608/bmfj.2025.384806.2409
Refaat, G., Zaki, M., Shenouda, H., Bayoumi, H. The Possible Ameliorating Effect of Lactoferrin as adjuvant to Ceftriaxone on Induced Intestinal Sepsis in adult male albino rats (Histological and Immunohistochemical Study). EKB Journal Management System, 2025; 42(8): 33-50. doi: 10.21608/bmfj.2025.384806.2409

The Possible Ameliorating Effect of Lactoferrin as adjuvant to Ceftriaxone on Induced Intestinal Sepsis in adult male albino rats (Histological and Immunohistochemical Study)

Benha Medical Journal
Volume 42, Issue 8, August 2025, Page 33-50  XML PDF (2.7 MB)
Document Type: Original Article
DOI: 10.21608/bmfj.2025.384806.2409
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Authors
Gehad Sherif Refaat email 1; Mohamed Magdi Zaki2; Helpies Delwar Shenouda3; Heba Elsayed Bayoumi4
1Demonstrator of histology and cell biology Faculty of Medicine - Benha University
2Professor of Histology and Cell Biology Faculty of Medicine - Benha University
3Asst. Professor of Histology and Cell Biology Faculty of Medicine - Benha University
4Asst. Professor of Histology and Cell Biology Faculty of Medicine - Benha University
Abstract
Background: Intra-abdominal infections can be seriously complicated to abdominal sepsis causing injury of various organs in the abdomen. Lactoferrin could be utilized in combination with Ceftriaxone to treat intestinal sepsis. Objective: to assess the possibility of Lactoferrin to ameliorate induced intestinal sepsis in adult male albino rats when used in combination with Ceftriaxone. Materials and methods: Fifty male rats were utilized in this study. The rats were divided into five groups: Group I (Control), Group II (Abdominal Sepsis model group): Rats received a single intraperitoneal injection of 1ml fresh fecal suspension (FSI) at a 50 mg/ml concentration. Group III (Abdominal Sepsis + Ceftriaxone group): Rats were treated with an intramuscular injection of Ceftriaxone, Group IV (Abdominal Sepsis + Lactoferrin group): Rats were treated with oral Lactoferrin, Group V (Abdominal Sepsis + Ceftriaxone + Lactoferrin group): Rats were treated with a combination of both. Colon samples were obtained and processed for histological examination. Results: Group II exhibited epithelial injury, detachment, and shedding; some crypts were damaged, obliterated, and lost parallelism; there was obvious depletion of goblet cells; and the lamina propria was markedly infiltrated with inflammatory cells. Furthermore, a significant increase in TNF-α, COX-2, and Caspase-3 immunoexpression was detected. Groups III and IV exhibited some histological attenuation, while group V showed histological architecture with more improvement. Conclusion:

Ceftriaxone can treat intestinal sepsis, but Lactoferrin can give better results when combined with Ceftriaxone.
Keywords
Alcian blue; TNF-α; COX-2; and Caspase-3
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