Biosynthesized AgO Nanoparticles from Probiotic Bacilli: Antibacterial Effect against Uropathogenic E. coli | ||
| Egyptian Journal of Medical Microbiology | ||
| Articles in Press, Accepted Manuscript, Available Online from 01 January 2026 | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| DOI: 10.21608/ejmm.2025.397328.1738 | ||
| Authors | ||
| Hussein Sarah* 1; Ammar R. Algburi2; Michael Chikindas3 | ||
| 1Department of Biotechnology, College of Science, University of Diyala, Baqubah 32001, Iraq; Department of Microbiology, College of Veterinary Medicine, University of Diyala, Baqubah 32001, Iraq | ||
| 2Department of Microbiology, College of Veterinary Medicine, University of Diyala, Baqubah 32001, Iraq | ||
| 3Department of Microbiology, College of Veterinary Medicine, University of Diyala, Baqubah 32001, Iraq Center for Agrobiotechnology, Don State Technical University, Rostov-on-Don, Russia; Health Promoting Naturals Laboratory, School of Environmental and Biological Sciences, Rutgers State University, New Brunswick, New Jersey, USA; I.M. Sechenov First Moscow State Medical University, Moscow, Russia | ||
| Abstract | ||
| Background: Silver oxide nano-particles (AgONPs) are produced using several protocols, including biological methods. The biological synthesis of NPs has emerged as a promising and eco-friendly approach. Objectives: The present study aimed to biosynthesize AgONPs (B-AgONPs) using cell-free supernatants of Bacillus mixed species (Bacillus subtilis, B. amyloliquefaciens, B. atrophaeus) and evaluate their antimicrobial potential against uropathogenic Escherichia coli. Methodology: The UV-visible spectrometer was used to analyze the optical properties that showed a peak at 420 nm. Additionally, X-ray diffraction (XRD) was followed to analyze the structural properties of nanoparticle crystals, and transmission Electron Microscopy (TEM) was utilized to detect the size, shape, and distribution of the synthesized nanoparticles. The B-AgONPs exhibited a spherical morphology with particle sizes ranging from 16 to 29.453 nm making them appropriate agents for biomedical applications. The multi-drug resistant uropathogenic E. coli isolates were identified and their antibiotic susceptibility was evaluated using VITEK® 2 compact system. Then, the antimicrobial activity of B-AgONPs was evaluated by well diffusion assay and broth-microdilutions to Identify the minimum inhibitory concentrations. Results: Our data showed that E. coli SR36 and SR69 growth was inhibited at B-AgONPs of 0.9 µg/mL and 1.9 µg/mL but with various growth survival compared to the control group. Low concentrations of B-AgONPs effectively inhibited the growth of uropathogenic E. coli isolated from humans and cattle. Conclusion: Based on our results, B-AgONP can be recommended as an alternative and highly effective antimicrobial drug that can be used in human and veterinary medicine against uropathogenic E. coli. | ||
| Keywords | ||
| Silver oxide nanoparticles; Biosynthesis; cell-free supernatant (CFS); Bacillus mix Antibacterial activity; Uropathogenic E. coli | ||
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