Silica Nanoparticles Biosynthesis from Saccharomyces sp. with antimicrobial activity | ||||
| Benha Journal of Applied Sciences | ||||
| Volume 10, Issue 2, February 2025, Page 17-27 PDF (552.3 K) | ||||
| Document Type: Original Research Papers | ||||
| DOI: 10.21608/bjas.2025.369447.1629 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Doaa Abd elhady Ahmed Osman  1; Mervat Gameel Hassan 2; Ahmed A. Hamed3; Mohamed. O. Abdel Monem4; Mahmoud S. A Shahin5
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| 1قسم النبات شعبة ميكروبيولوجي ـــ كلية العلوم ـــ جامعة بنهاــ مدينة بنها ـــ مصر | ||||
| 2Botany and Microbiology Department, Faculty of Science, Benha University, Egypt | ||||
| 3Microbial Chemistry department , National Research Centre , El Buhouth St. 33, Cairo 12622, Egypt | ||||
| 4Professor of Botany and Microbiology Department , Faculty of Science, Benha University | ||||
| 5Main laboratories for Egyptian Army | ||||
| Abstract | ||||
| The present study focuses on the isolation of Saccharomyces spp. from bakery yeast samples and their potential for biosynthesizing silica nanoparticles (SiNPs) with antibacterial properties. Yeast samples were collected from four different bakeries in Benha to ensure strain diversity, and multiple isolates were successfully obtained. Screening for silica nanoparticle biosynthesis revealed significant variations among the isolates, with DO1S4 and DO4S1 exhibiting the highest biosynthesis potential (+++), indicating strong enzymatic activity and effective interaction with silica precursors. Moderate biosynthesis (++) was observed in isolates DO1S1 and DO2S3, while several isolates showed weak or no biosynthesis activity. The antibacterial activity of SiNPs synthesized from the highly active strain DO1S4 was evaluated against Escherichia coli and Staphylococcus aureus. The biosynthesized SiNPs demonstrated significant antibacterial effects, with inhibition rates of 77.025% against E. coli and 52.02% against S. aureus. However, their efficacy was lower than ciprofloxacin (5 µg/mL), which exhibited inhibition rates of 99.05% and 98.25%, respectively. The enhanced effect against E. coli compared to S. aureus is likely due to differences in bacterial cell wall structures, affecting nanoparticle penetration and antimicrobial efficiency. The antibacterial activity of the biosynthesized SiNPs is attributed to mechanisms such as reactive oxygen species (ROS) generation, | ||||
| Keywords | ||||
| Silica Nanoparticles; Saccharomyces sp; antimicrobial activity | ||||
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