Cytotoxicity and Antioxidant Potential of Vanillin from Coffee Peel Extract and Sorafenib in human MDAMB-231 Breast Cancer cells: A Potential Therapeutic strategy
El-sayed, S., Hassan, M., O. Abdel Monem, M., Shahin, M., hassan, J., Elmetwalli, D. (2025). Cytotoxicity and Antioxidant Potential of Vanillin from Coffee Peel Extract and Sorafenib in human MDAMB-231 Breast Cancer cells: A Potential Therapeutic strategy. EKB Journal Management System, 10(2), 45-49. doi: 10.21608/bjas.2025.370889.1645
shimaa sobhy El-sayed; Mervat Gameel Hassan; Mohamed. O. Abdel Monem; Mahmoud S. A Shahin; jihan hassan; Dr. Alaa Elmetwalli. "Cytotoxicity and Antioxidant Potential of Vanillin from Coffee Peel Extract and Sorafenib in human MDAMB-231 Breast Cancer cells: A Potential Therapeutic strategy". EKB Journal Management System, 10, 2, 2025, 45-49. doi: 10.21608/bjas.2025.370889.1645
El-sayed, S., Hassan, M., O. Abdel Monem, M., Shahin, M., hassan, J., Elmetwalli, D. (2025). 'Cytotoxicity and Antioxidant Potential of Vanillin from Coffee Peel Extract and Sorafenib in human MDAMB-231 Breast Cancer cells: A Potential Therapeutic strategy', EKB Journal Management System, 10(2), pp. 45-49. doi: 10.21608/bjas.2025.370889.1645
El-sayed, S., Hassan, M., O. Abdel Monem, M., Shahin, M., hassan, J., Elmetwalli, D. Cytotoxicity and Antioxidant Potential of Vanillin from Coffee Peel Extract and Sorafenib in human MDAMB-231 Breast Cancer cells: A Potential Therapeutic strategy. EKB Journal Management System, 2025; 10(2): 45-49. doi: 10.21608/bjas.2025.370889.1645

Cytotoxicity and Antioxidant Potential of Vanillin from Coffee Peel Extract and Sorafenib in human MDAMB-231 Breast Cancer cells: A Potential Therapeutic strategy

Benha Journal of Applied Sciences
Volume 10, Issue 2, February 2025, Page 45-49  XML PDF (544.2 K)
Document Type: Original Research Papers
DOI: 10.21608/bjas.2025.370889.1645
View on SCiNiTO View on SCiNiTO
Authors
shimaa sobhy El-sayed1; Mervat Gameel Hassanorcid 2; Mohamed. O. Abdel Monem email 3; Mahmoud S. A Shahin4; jihan hassanorcid 5; Dr. Alaa Elmetwalliorcid 6
1Botany and Microbiology department , faculty of science , Benha university , Benha 33516
2Botany and Microbiology Department, Faculty of Science, Benha University, Egypt
3Professor of Botany and Microbiology Department , Faculty of Science, Benha University
4Main laboratories for Egyptian Army
5Applied Medical Chemistry Medical Research Institute Alexandria university
6Sherbin, Mansoura, Egypt
Abstract
Breast cancer (BC) is the most prevalent malignancy among women in Egypt, accounting for 34.9% of all female cancer cases. Given the frequent limitations of conventional treatments, the need for alternative therapeutic strategies remains critical. Vanillin, naturally occurring phenolic compound, has demonstrated multiple bioactive properties, including anti-inflammatory, anticancer, and antioxidant effects. Objective:This study aimed to assess the cytotoxic effects of vanillin derived from Coffee Peel Extract and sorafenib (SOR), an anti-hepatocellular carcinoma drug, using the triple-negative breast cancer(TNBC) MDA-MB-321 cell line. Additionally, we investigated their individual and combined impact on lipid peroxidation and antioxidant enzyme activity.Methods:MDA-MB-321 cells were treated with varying concentrations of vanillin (50–200 µM) and SOR(6.25–100 µM)for 48 hours, after which cell viability was assessed using the MTT assay.The activity of superoxide dismutase (SOD) and catalase(CAT)—key antioxidant enzymes—was quantified using colorimetric assays, while malondialdehyde (MDA) levels, a biomarker of lipid peroxidation, were also measured. Results:Both SOR and vanillin significantly (P < 0.001) reduced MDA-MB-321 cell viability.However, SOR exhibited greater cytotoxicity (IC90 = 144.8 ± 5.1µg/ml) compared to vanillin(IC90 = 255.4 ± 6.5 µg/ml).In comparison to untreated and DMSO-treated control cells, SOR treatment alone resulted in the highest SOD and CAT activity,whereas the combination of SOR and vanillin led to the lowest MDA levels, indicating reduced oxidative stress. Conclusion:These findings suggest that SOR and vanillin exert anticancer effects inTNBC cells by modulating lipid peroxidation and antioxidant defense mechanisms. This study provides insight into their potential mechanism of action, supporting further exploration of vanillin-based combination therapies for breast cancer treatment.
Keywords
vanillin; oxidative stress; cytokine; antioxidants
Statistics
Article View: 80
PDF Download: 36