Association of IL-33 Gene Polymorphisms with Recurrent Pregnancy Loss in Iraqi women: A Case-Control Study | ||
| Egyptian Journal of Medical Microbiology | ||
| Article 56, Volume 35, Issue 1, January 2026 | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| DOI: 10.21608/ejmm.2025.401013.1760 | ||
| Authors | ||
| Mohammed K. Mutasher* ; Taghreed F. Almahbobi; Ahmed M, Issa | ||
| Medical Microbiology Department, Faculty of Medicine, Jabir Ibn Hayyan University for Medical and Pharmaceutical Sciences, Iraq | ||
| Abstract | ||
| Background: The term "recurrent pregnancy loss" (RPL) describes two or more consecutive abortions performed prior to the 20th week of pregnancy. RPL may be significantly influenced by an imbalance of inflammatory substances, such as interleukins (IL33). Investigating the relationship between the rs16924159 polymorphism in the interleukin-33 (IL33) gene and RPL in Iraqi women was the goal of this study. The cytokine interleukin-33 (IL33) belongs to the interleukin-1 family and attaches to the unique ST2 receptor. The aim of the study was to evaluate the effects of IL-33 levels and the IL33 gene polymorphism rs16924159 on recurrent pregnancy loss. Methodology: In this case-control study, 60 women with RPL served as the case group, while 60 healthy controls gave birth without incident. Using the salting-out approach, genomic DNA was isolated from entire blood samples. The rs16924159 polymorphism fragments were amplified by PCR, and DNA TETRA-ARMS was used for genotyping. IL-33 was measured using the ELISA technique on serum blood samples. Results: According to the data, the case group's frequency of the AA genotype of the rs16924159 polymorphism was significantly greater than that of the healthy controls (p = 0.0009), and IL-33 was substantially higher in the control group than in the case group (p = 0.001). Conclusions: Overall, we demonstrated that the rs16924159 polymorphism in the IL-33 gene and the level of IL-33 may be a significant factor in the risk of RPL among Iraqi women. However, more research on various racial and geographic groups may be helpful in determining how the rs16924159 polymorphism affects RPL. | ||
| Keywords | ||
| IL-33; polymorphism; recurrent pregnancy loss | ||
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