The probiotic (Lactobacilli and bifidobacteria) effect on multi-drug resistant Pseudomonas aeruginosa in different clinical isolates | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 21 July 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2025.395098.2910 | ||||
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| Authors | ||||
Shaimaa H Zaki   1; Dalia N. Kotb1; Haitham Abdelhamid2; Nehal Refaat Raouf3; Marian Refaat Raouf1
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| 1Medical Microbiology and Immunology Department, Faculty of Medicine, Minia University, Minia, Egypt | ||||
| 2Tropical Medicine Department., Faculty of Medicine, Minia University, Minia, Egypt | ||||
| 3Public Health and Preventive Medicine, Faculty of Medicine, Minia University, Minia, Egypt. | ||||
| Abstract | ||||
| Background: The emergence and wide spread of multidrug-resistant (MDR) Pseudomonas aeruginosa strains have recently become an important public health problem that necessitates study and trial of probiotics as a new alternative strategy to fight against these resistant bacteria. This study aimed to evaluate the effect of probiotics against the growth and biofilm activity of P. aeruginosa strains isolated from patients with urinary tract infections and pneumonia. Methods: A total of 120 P. aeruginosa strains were isolated and confirmed by detection of oprL gene by PCR, then tested for antimicrobial resistance by disk diffusion method. The effect of probiotics alone (lactobacillus and Bifidobacterium) as well as effect of antibiotic/ probiotic combinations on growth of MDR isolates was tested by agar well diffusion. Biofilm production was tested by microtiter plate method before and after adding probiotics. Results: Forty-one (34.1%) P. aeruginosa isolates showed multidrug resistance. By agar well diffusion, 70.7% of isolates showed growth inhibition after adding probiotics alone. Addition of antibiotic/probiotic combinations significantly enhanced the means of inhibition zone diameters for most antimicrobial agents (p-values <0.001) in MDR strains; the best enhancement was for Meropenem (81.8%) followed by Piperacillin (56.2%) and Ceftazidime (53.3%) Colistin (46.2%), cefepime (37.5%), Levofloxacin (33.3), gentamicin (28.6%) and Amikacin (18.8%) while no enhancement observed for piperacillin-tazobactam. There was a 48.75% reduction in biofilm production after adding probiotics (p-value <0.001). Conclusions: Probiotics can be used in the treatment of MDR P. aeruginosa as they significantly inhibited growth and biofilm activity of these strains | ||||
| Keywords | ||||
| Keywords: Pseudomonas aeruginosa; MDR; probiotic; Biofilm | ||||
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