Investigating Virulence and Multidrug Resistance Capability of Klebsiella Pneumoniae Isolates From Karish "Soft" Cheese and Chicken Carcasses Sold in Qena City, Egypt | ||
| Egyptian Journal of Veterinary Sciences | ||
| Articles in Press, Corrected Proof, Available Online from 22 July 2025 PDF (980.58 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejvs.2025.378956.2808 | ||
| Authors | ||
| Basma Gamal1; Noura F Mostafa2; Aya seleem3; Ghada Ibrahim4; Sally Sakr5; Sahar Mohamed6; Asmahan Ali6; Abeer Afifi6; Eman Ezzat7; Mustafa Sadek* 1 | ||
| 1Department of Food Hygiene and Control, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt | ||
| 2Gastro-Enterology Surgery Center (GEC), Mansoura University, Mansoura | ||
| 3Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University | ||
| 4Agricultural Research Center (ARC), Animal Health Research Institute (AHRI), Bacteriology De-partment, Ismailia Branch, 41511, Egypt | ||
| 5Department of Food Science and Human Nutrition, College of Agriculture and Food, Qassim Uni-versity, Buraydah 51452, Saudi Arabia Dairy Science Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt | ||
| 6Department of Food Science and Human Nutrition, College of Agriculture and Food, Qassim Uni-versity, Buraydah 51452, Saudi Arabia | ||
| 7Faculty of Veterinary Medicine (Hygiene and food control), King Salman International University, Ras Sudr, Egypt | ||
| Abstract | ||
| Klebsiella pneumoniae significantly contributes to antimicrobial-resistant infections in humans, posing a considerable risk to both human and animal health worldwide, so this study was designed to identify the virulence and antimicrobial resistance profile of K. pneumoniae isolated from chicken carcasses and raw milk soft "Karish" cheese in Qena city, Egypt. One hundred food samples, 50 samples of each were examined for K. pneumoniae presence. A total of only seven isolates were confirmed to be K. pneumoniae through biochemical tests. PCR was performed for several virulence genes (iutA, kfu and rmpA genes) and antimicrobial resistance genes (mcr-1, blaSHV, blaCTX-M and blaCMY-2 genes). In total, 7% of food samples tested positive for K. pneumoniae, one isolate from chicken carcasses and six from Karish cheese samples. All isolates exhibited resistance to amoxicillin, ceftazidime and amoxicillin-clavulanic acid, while they were susceptible to imipenem, gentamicin, tobramycin, cefotaxime, aztreonam and meropenem. K. pneumoniae isolates from Karish cheese samples showed susceptibility to imipenem (100%), gentamicin and tobramycin (83.33%), while (100%) resistance was displayed against amoxicillin, ceftazidime and amoxicillin clavulanic acid. The most predominant virulence gene was iutA (100%), followed by kfu (85.7%). All K. pneumoniae isolates were found to carry blaSHV. Five mcr-1 and two blaCTX-M-producing K. pneumoniae isolates were recovered, while blaCMY-2 was absent in all isolates. Our results raising serious concerns as K. pneumoniae poses significant public health risks because consumption of such products may transmit resistant strains to humans through the food chain, so stricter regulations are required to ensure food safety. | ||
| Keywords | ||
| Food safety; K. pneumoniae; virulence; antimicrobial resistance | ||
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