Detection and molecular characteristics of methicillin resistant staphylococcus aureus MRSA in some meat | ||
Menoufia Veterinary Medical Journal | ||
Volume 1, Issue 1, August 2025, Pages 1-12 PDF (945.55 K) | ||
Document Type: Original Article | ||
DOI: 10.21608/vmmj.2023.243304.1004 | ||
Author | ||
Shireen Mohammed Kozaa* | ||
قسم البكتيريا والفطريات والمناعة كلية الطب البيطري جامعة المنوفية | ||
Abstract | ||
Food poisoning is one of the most important problems that can affect large part of population. Staphylococcus aureus (S. aureus) is one of the main causes of bacterial food poisoning. A hundred random samples of burger, chicken nuggets, luncheon and kofta (25 of each) obtained from retail outlets were examined bacteriologically for the occurrence of methicillin resistance Staphylococcus aureus (MRSA). A total of 25 S.aureus isolates was recovered from - burger 9/25 (36%), chicken nuggets 7/25 (28%), luncheon 6/25(24 %) and kofta 3/25 (12%) Application of antibiotic sensitivity test on isolated S.aureus cleared that the highest resistance was exhibited against penicillin (96%) followed by flucloxacillin (92%), cefotaxime (68%), erythromycin (60%), clindamycin (61.54%), linezolid (36%) and vancomycin (28%) , using Polymerase chain reaction (PCR) on S.aureus to detect their virulence and resistance genes Hemolysin virulence gene (hlg) which was detected in (50%) of the examined isolates, while beta- lactam resistance gene (blaZ) and met hicillin resistance gene (mec A) were found in (50%) and (100%) of tested S.aureus, respectively. KEYWORDS: S.aureus, Burger, meat products, Resistant genes. | ||
Keywords | ||
S.aureus; burger; resistant genes; meat products | ||
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