Prevalence of Feline Calicivirus and Feline Herpesvirus -1 among Domestic Cats in Three Egyptian Governorates | ||||
Egyptian Journal of Veterinary Sciences | ||||
Articles in Press, Accepted Manuscript, Available Online from 22 July 2025 PDF (825.74 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ejvs.2025.386486.2858 | ||||
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Authors | ||||
Abeer Mohamed Hagag1; Noura Fysal Alkhalefa1; Alaa Mohamed Abdelatty2; Bassiouni Abdelkader Heleil3; Asmaa Fawzy Magouz ![]() | ||||
1Department of Virology,Fac of Vet Med ,Kafrelsheikh University, Egypt | ||||
2Department of Pathology,Fac of Vet Med ,Kafrelsheikh University, Egypt | ||||
3Department of Theriogenology,Fac of Vet Med ,Kafrelsheikh University, Egypt | ||||
Abstract | ||||
Upper respiratory tract infections are major causes of feline morbidity and mortality. This study describes the prevalence of Feline herpesvirus type 1 (FHV-1) and Feline calici virus (FCV) among domestic cats in three Egyptian governorates. One hundred and twenty conjunctival or nasal swabs were obtained from diseased cats from private pet animal clinics and shelters located in three Egyptian provinces (Cairo, El Gharbia, and Kafrelsheikh). FHV-1 isolation was done in the Chorioallantoic membranes (CAMs) of 13-days-old SPF eggs. Infected CAMs showed large white pock lesions. Polymerase chain reaction (PCR) of the thymidine kinase gene (TK) of FHV-1 was positive in 34/120 (28.3%) of the suspected cases. In comparison, PCR amplification of ORF2 and ORF3 of FCV was successful in 32/120 (26.6%) of the suspected cases. Mixed infection with the two viruses were reported in 12/120 (10%) of the cases. Two FHV1 genes were sequenced, and the TK gene sequences were identical to globally strains in the GenBank. For FCV one of the amplified genes were sequenced and the ORF2 and ORF3 genes sequences of the FCV isolates shared 81-87% similarity with other reference strains in the GenBank. | ||||
Keywords | ||||
Feline Herpesvirus 1; Feline Calicivirus; Cats; PCR; Egypt | ||||
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