Molecular Characterization and Antibiotic Resistance Profiling of Clostridium perfringens Isolated From Broiler Chickens | ||||
| Egyptian Journal of Veterinary Sciences | ||||
| Articles in Press, Corrected Proof, Available Online from 22 July 2025 PDF (890.08 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejvs.2025.392160.2893 | ||||
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| Authors | ||||
Aya Auob  1; Rasha Elkenany2; Amal Awad 3; Gamal Younis4
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| 1Departmant of Bacteriology, lmmunology, and Mycology Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt. | ||||
| 2Department of Bacteriology, Immunology, and Mycology Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt. | ||||
| 3Department of Bacteriology, Mycology, and Immunology, Faculty of veterinary medicine, Mansoura University, Mansoura 35516, Egypt | ||||
| 4Bacteriology, Mycology, and Immunology Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt. | ||||
| Abstract | ||||
| Clostridium Perfringens (C. perfringens) is a global bacteria disease has ability to produce various toxins, contributing significantly to gastrointestinal diseases in poultry and posing public health risks. The objective of this study was to investigate the prevalence of Clostridium perfringens in broiler chickens. Particular emphasis was placed on the characterization of toxinotypes and the molecular detection of genes associated with antibiotic resistance to commonly utilized antibiotic agents. Out of 265 collected samples, 50 were confirmed as C. perfringens through both biochemical and molecular techniques. Toxin gene detection revealed that 100% of the isolates carried the cpa gene, while 30% harbored cpb2 and 22% were positive for the netB gene, indicating their involvement in disease pathogenesis. Antibiotic susceptibility testing revealed 100% resistant to penicillin, ampicillin, oxacillin, amoxicillin-clavunic acid, streptomycin, erythromycin, tetracycline, and ofloxacine followed by gentamicin (96%), tylosin (72%), and ciprofloxacin (60%). Molecular analysis detected resistance genes with the following prevalence: ermB (100%), tetA (80%), tetM (60%), blaTEM (70%), blaSHV (28%), blaOXA10 (12%), aphA1 (64%), qnrS (46%), qnrA (40%), and qnrB (22%). The results demonstrate that C. perfringens isolates from diseased broilers carry a high burden of toxin and antibiotic resistance genes. Understanding the genetic basis will aid in developing better control strategies and preserving antibiotic efficacy. This study provides essential data on the molecular characteristics of C. perfringens in poultry, highlighting the need for improved biosecurity, ongoing surveillance programs, and rational antibiotic use to reduce the spread of virulent and multidrug-resistant strains. | ||||
| Keywords | ||||
| C. perfringens; toxin genes; antibiotic susceptibility; resistance genes profiling; broiler chickens | ||||
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