Antimicrobial and antibiofilm potential of silver nanoparticles fabricated with Klebsiella variicola against four foodborne pathogens | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 22 July 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2025.390788.2846 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Khnsaa Basem Fadhil1; Mohammed Hasan Mohammed1; Rana Naseer Abdul Hameed1; Helal Moussa   2
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| 1Al-Dour Technical Institute, Northern Technical University, Iraq | ||||
| 2Radioisotope Department, Nuclear Research Center, Atomic Energy Authority, Egypt. | ||||
| Abstract | ||||
| Background: Foodborne infections are a serious concern to public health, especially because they can create biofilms that increase their resistance to standard antibiotic therapies. Aim: the study lies in the development and application of ecofriendly, biogenic silver nanoparticles (Ag-NPs) synthesized using a bacterial strain, Klebsiella variicola, for inhibiting growth of foodborne pathogens and their biofilms. Methods: In the current study, the endophytic bacterial strain Klebsiella variicola was used to create ecofriendly silver nanoparticles (Ag-NPs), which were then examined using TEM, XRD, ATR-FTIR, and UV-VIS spectroscopy. The resultant Ag-NPs, which ranged in size from 5-15 nm, showed a distinct spherical crystalline structure. Results: The antimicrobial performance of biogenic Ag-NPs was evaluated against four foodborne pathogens (Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa, and Staphylococcus aureus), demonstrating a dose-dependent bactericidal effect, with low MIC values (25-30 µg/mL). Furthermore, Ag-NPs (10 µg/mL) effectively suppressed biofilm formation by E. coli, B. cereus, P. aeruginosa, and S. aureus, with inhibition ratios of 10.33±2.05%, 15.33±1.24%, 25.3±2.8%, and 34.3±2.8 % respectively. Notably, the highest suppression was observed in E. coli, followed by P. aeruginosa, B. cereus, and S. aureus. Furthermore, the anti-adherence properties of Ag-NPs exhibited a dose-dependent effect, with the highest biofilm inhibition observed at 20 µg/mL. At this concentration, biofilm suppression ratios were 84.33±3.68% for B. cereus, 82.33±3.6% for P. aeruginosa, and 86.33±3.6% for E. coli. Conclusions: The study demonstrated the successful green synthesis of silver nanoparticles (Ag-NPs) using the endophytic bacterium Klebsiella variicola. The results demonstrate the potential of biogenic Ag-NPs in exhibiting significant antimicrobial and antibiofilm activity against foodborne pathogens, suggesting their potential as a fruit preservative and a possible alternative therapy for multidrug-resistant infections. | ||||
| Keywords | ||||
| Antimicrobial; Silver nanoparticles; Antibiofilm; Foodborne pathogens; Alternative antimicrobial therapy | ||||
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