Purslane Seed Extract Mitigates Thioacetamide-induced Acute Kidney Injury in Rats by Suppressing Renal Necroptosis and Inflammation | ||
| Journal of Advanced Pharmacy Research | ||
| Article 5, Volume 9, Issue 3, July 2025, Pages 148-156 PDF (670.36 K) | ||
| Document Type: Research Article | ||
| DOI: 10.21608/aprh.2025.386728.1317 | ||
| Authors | ||
| Shimaa O. Ali* 1; Ibrahim S. Kamel2; Asmaa A. ElMonier1 | ||
| 1Department of Biochemistry, Faculty of Pharmacy, Cairo University, Cairo, Egypt | ||
| 2Department of Chemistry, Faculty of Science, Port Said University, Port Said, Egypt | ||
| Abstract | ||
| Background: Acute kidney injury (AKI), a global public health challenge with limited treatment options, involves necroptosis and inflammation as key pathological mechanisms. Purslane seed extract (PSE), known for its antioxidant and anti-inflammatory properties, may offer therapeutic potential, but its mechanisms in AKI remain unexplored. Methods: A rat model of thioacetamide (TAA)-induced AKI was used to evaluate PSE’s renoprotective effects. Rats were pretreated with PSE (400 mg/kg) for 15 days before TAA single dose administration (300 mg/kg). Serum biomarkers (creatinine, BUN, KIM-1, NGAL, TNF-α) and renal tissue markers (RIPK1, RIPK3, MLKL, ZBP1, HMGB1) were analyzed. Histopathological examination of kidney tissues was also performed. Results: PSE pretreatment restored renal function by significantly reducing serum creatinine and BUN. PSE attenuated tubular injury, lowering KIM1 and NGAL levels and inhibited necroptosis, via suppressing renal levels of RIPK1, RIPK3, and MLKL. It also reduced renal ZBP1 concentration, indicating blockade of non-canonical necroptosis and diminished inflammation through decreasing TNF-α and HMGB1 levels. PSE pretreatment also managed to alleviate the histopathological changes caused by AKI. Conclusion: PSE protects against TAA-induced AKI by dual inhibition of RIPK1-dependent and ZBP1-dependent necroptosis pathways, coupled with anti-inflammatory effects. These findings highlight PSE as a promising multi-targeted natural therapy for AKI, warranting further clinical exploration. | ||
| Keywords | ||
| Acute kidney injury; Purslane seed extract; Necroptosis; ZBP1; RIPK1/MLKL | ||
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