Molecular study of hypervirulent Klebsiella pneumoniae isolated from different clinical samples | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 24 July 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2025.400825.2990 | ||||
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| Authors | ||||
Mustafa Mahmoud Al-Qaisi  ; Raghad Hassan Hussein ; Luma Yousif Mahdi
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| Department of Medical Laboratory Techniques, College of Health and Medical Techniques, Middle Technical University, Baghdad, Iraq | ||||
| Abstract | ||||
| Background: Recently Hypervirulent Klebsiella pneumoniae (HvKp) considered as a superbug strains is characterized by high pathogenicity and mortality (hypervirulence). Objective: This study aims to molecularly characterize Iraqi HvKp isolates and confirm the role of hypermucoviscosity and sidersphores in HvKp. Methods: one hundred isolates of Klebsiella pneumoniae (Kp) from (urine, abscess, wound, and sputum) specimens collected from different Hospitals. These isolates were identified by selective media and VITEK-2 system. The hypermucoviscosity feature of HvKp was identified using string test. Phenotypic detection of ESBL-producing HvKp were done using CHROM agar. Antimicrobial susceptibility was tested against 10 antibiotics. The identification of rmpA, iucA, and peg344 was performed using polymerase chain reaction. Results: Of the 100 isolates, 67% of them were HvKp and 33% were classical Klebsiella pneumoniae (CKp). Among HvKp isolates, 46/67(68.7%) were ESBL positive, 64/67(95.5%) of them were revealed multidrug resistant (MDR) pattern, and the resistance rates of most studied antibiotics were higher in HvKp than in CKp isolates. Regarding the virulence genes, rmpA was the most prevalent among the HvKp isolates (89.6%), followed by peg-344 (80.6%), and iucA(62.7%) genes. Conclusion: The study concluded that the HvKp displayed an increasing prevalence and virulence highlighting an increase in the emergence and spread of this superbug strains. | ||||
| Keywords | ||||
| Hypervirulence Klebsiella pneumoniae; hypermucoviscosity; siderophores; ESBL; virulence plasmid | ||||
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