Effect of Naloxone Pretreatment on Rat suprarenal gland after Hepatic Ischemia–Reperfusion injury | ||
| Minia Journal of Medical Research | ||
| Articles in Press, Accepted Manuscript, Available Online from 27 July 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mjmr.2025.399873.2012 | ||
| Authors | ||
| Abdel Hamid Sayed AboBakr1; samah mohamed mahmoud2; Nashwa Fathy El-Tahawy3; Rania Nady Nour ElDeen* 4; Medhat Ata5 | ||
| 1Assistant professor of Anatomy and Embryology, Faculty of Medicine, Minia University, Egypt | ||
| 2anatomy department,faculty of medicine,Minia university,Minia,Egypt | ||
| 3Faculty of Medicine, Minia University | ||
| 4Anatomy, Faculty of Medicine, Minia University, Minia, Egypt | ||
| 5Assistant professor of Anatomy and Embryology Faculty of Medicine, Minia University | ||
| Abstract | ||
| Introduction: The adrenal gland is a vital endocrine organ responsible for secreting adrenal hormones. One clinically significant condition is hepatic ischemia–reperfusion (IR) injury, which can lead to dysfunction in multiple distant organs. Naloxone is potent antioxidant Aim: This study aimed to explore the potential remote effects of hepatic IR injury on the structural integrity of the adrenal cortex. Materials and Methods: The study included twenty male Sprague-Dawley albino rats that were healthy and had an average age of 6-8 weeks. The rats were divided into four groups, with ten rats in each: the control, sham control group, the ischemia-reperfusion and naloxone pretreated group. This study examined the levels of corticosterone, aspartate transaminase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH), and other enzymes in the blood. Additionally, the antioxidant enzyme catalase and malondialdehyde (MDA) were assessed. The adrenal glands were prepared for microscopic analysis after they were taken. Results: When comparing the IR group to the SC group, the results showed that serum levels of liver enzymes (AST, ALT, and LDH), corticosterone, MDA, and TNF-α were significantly higher, whereas catalase levels were significantly lower. In the IR group, adrenal cortex histology revealed architectural disruption. Extensive cytoplasmic vacuolization was seen in the enlarged and degraded cells of the zona fasciculata and zona glomerulosa.. Small numbers of cells in the zona reticularis exhibited cytoplasmic vacuoles and black nuclei. Naloxone pretreatment mitigates these effects Conclusion: These findings indicate that hepatic IR injury adversely affects the structural integrity of the adrenal cortex. | ||
| Keywords | ||
| adrenal cortex; hepatic ischemia–reperfusion; rat | ||
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