ENHANCING BONE DEFECT HEALING IN RABBITS BY USING AUTOLOGOUS BONE MARROW ASPIRATE AND ELECTRICAL STIMULATION | ||||
| Assiut Veterinary Medical Journal | ||||
| Article 18, Volume 71, Issue 186, July 2025, Page 228-241 PDF (1.36 MB) | ||||
| Document Type: Research article | ||||
| DOI: 10.21608/avmj.2025.329315.1439 | ||||
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| Authors | ||||
SOZAN ALI MUHAMAD 1; FAISAL R. KHALID1; HANA H. MUSTAFA 1; OTHMAN JALAL ALI  2; DEKAN A. RADAH 1
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| 1Department of Surgery and Theriogenology, College of Veterinary Medicine, University of Sulaimani, New Sulaimani, Street 27, Sulaimani City, Kurdistan Region, Northern Iraq. | ||||
| 21 Department of Surgery and Theriogenology, College of Veterinary Medicine, University of Sulaimani, New Sulaimani, Street 27, Sulaimani City, Kurdistan Region, Northern Iraq. and 2 Department of Anesthesia, College of Health Sciences, Cihan University Sulaimaniya, Sulaimaniya, Kurdistan Region, Iraq. | ||||
| Abstract | ||||
| Electrical stimulation can promote bone formation by stimulating cellular activity, enhancing the proliferation and differentiation of osteoprogenitor cells, increasing local blood flow, and upregulating the expression of bone-related genes and proteins. When combined with BMA, they further contribute to bone healing by promoting osteogenesis, osteo-induction, and modulating the local healing environment. In this experiment, 36 rabbits were used, and they were randomly divided into three groups (CG = Control, BM= Bone Marrow and BME = Bone Marrow + Electrical stimulation). Under general anesthesia, all animals underwent osteotomy and a small defect up to 2 mm was created in the fibular bone using a fine electrical rotating saw. After osteotomy, group CG was subjected to percutaneous injection of 2 ml of normal saline at the fracture site. Group BM was subjected to percutaneous injection of 2 ml of autologous bone marrow. Group BME was subject to injection of 2 ml of autologous bone marrow percutaneously and electrical stimulation at the fracture site three days after osteotomy, using a capacitive coupling device electrical stimulator with a power source of 9 volts and an output of 30 KHz, for 30 minutes twice daily for four weeks. This experiment was assessed by clinical, radiographical, postmortem, and histopathological examinations at the 2nd, 4th, 6th, and 8th postoperative weeks. In conclusion, BME group rabbits demonstrated excellent healing signs, almost starting from the second post-operative week. The defects restored its normal anatomical shape with a high degree of remodeling process compared to groups CG and BM. | ||||
| Keywords | ||||
| Osteotomy; Electrical stimulation; Bone growth; Rabbit; Fibula | ||||
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