Field Application of PEGylated Zinc Oxide Nanoparticles on Infected Cattle with Methicillin-Resistant Staphylococcus Aureus (MRSA)

Hamouda, Ramzy Hamouda; Allam, Hanaa Abdelazeem; Khalil, Samer Foad; Mahmoud, Marwa Fathy

doi:10.21608/ejvs.2025.381885.2825

	Field Application of PEGylated Zinc Oxide Nanoparticles on Infected Cattle with Methicillin-Resistant Staphylococcus Aureus (MRSA)
Egyptian Journal of Veterinary Sciences
Articles in Press, Corrected Proof, Available Online from 28 July 2025 PDF (922.18 K)
Document Type: Original Article
DOI: 10.21608/ejvs.2025.381885.2825
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Authors
Ramzy Hamouda Hamouda¹; Hanaa Abdelazeem Allam¹; Samer Foad Khalil²; Marwa Fathy Mahmoud ³
¹Mastitis and Neonatal Disease Department at Animal Reproduction Research Institute (ARRI), Agriculture Research Center (ARC), Giza, Egypt.
²Immunological Unit at Animal Reproduction Research Institute (ARRI), Agriculture Research Center (ARC), Giza, Egypt.
³Animal Reproduction Research Institute at Agriculture Research Center
Abstract
The recurrence of infectious diseases and the continued development of resistance among pathogenic bacteria, such as Staphylococcus aureus, pose a serious threat to global public health. Therefore, the search for novel treatments focused on combating antibiotic resistance has become essential. In this study, 13 antibiotic tablets (levofloxacin 5 µg, gentamicin 10 µg, methicillin 10 µg, linezolid 30 µg, chloramphenicol 30 µg, sulfamethoxazole 1.25 µg, azithromycin 15 µg, tetracycline 30 µg, ciprofloxacin 5 µg, cefoxitin 30 µg, amicolin 30 µg, vancomycin 30 µg, and amoxicillin 30 µg) and 7 concentrations of zinc oxide nanoparticles (ZnO-NPs) were used against 6 Egyptian isolates of methicillin-resistant Staphylococcus aureus (MRSA). The cytotoxicity of these particles was studied. On VERO cells, cell viability ranged from 80% to 200%, and the antibacterial efficacy of ZnO-NPs was superior to that of all antibiotic tablets. In this study, 10 ml (500 ng) of ZnO-NPs was used as a treatment for each of the five cows with mastitis caused by S. aureus., compared to intramammary antibiotics (cephalexin 200 mg and kanamycin monosulfate 100,000 IU/10 ml) used in two infected cows. ZnO-NPs showed a cure after 24 hours, and no recurrence of the infection was confirmed, and no residual ZnO-NPs were found in the milk or any alteration in its contents.
Keywords
ZnO NPs; antimicrobial; MRSA; treatment; mastitis


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