BACTERIOLOGICAL AND MOLECULAR STUDIES ON PSEUDOMONAS AERUGINOSA ISOLATED FROM AFRICAN SHARPTOOTH CATFISH AND NILE TILAPIA | ||||
| Assiut Veterinary Medical Journal | ||||
| Article 30, Volume 71, Issue 186, July 2025, Page 372-384 PDF (779.21 K) | ||||
| Document Type: Research article | ||||
| DOI: 10.21608/avmj.2025.346209.1532 | ||||
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| Authors | ||||
EMAN, M.A. EL-RAIS  1; ASHRAF, A. ABD EL TAWAB 1; AHMED A. A. MAAROUF 2; AMIRA M. RIZK 1
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| 1Bacteriology, Immunology and Mycology Department, Faculty of Veterinary Medicine, Benha University, Egypt. | ||||
| 2Animal Health Research Institute "Benha Branch, Egypt | ||||
| Abstract | ||||
| Pseudomonas aeruginosa is a Gram-negative bacterium commonly found in aquatic environments. It poses a serious threat to aquaculture due to its opportunistic pathogenicity and causes severe infections, high mortality rates in fish populations. The present investigation involved 250 organ samples isolated from lesions in the muscle, liver, kidney, intestine, and spleen of diseased Nile tilapia and African sharptooth catfish of different sizes. The fish were collected from various fish markets in Qalyubia Governorate, Egypt, between September 2023 and February 2024 for isolation, phenotypic and genotypic characterization of Pseudomonas aeruginosa (Ps. aeruginosa). The results showed that 52 Ps. aeruginosa isolates were recovered from fish organs, and they were isolated from 25 muscle samples (48.1%), followed by intestine 13 (25%), then liver 8 (15.4%), kidney 5 (9.6%), and one spleen sample from African sharptooth catfish (1.9%). The disc diffusion test revealed multidrug resistance (MDR) in all isolates against the used antimicrobial agents. The isolated Ps. aeruginosa were highly sensitive to norfloxacin, followed by gentamicin and ciprofloxacin. In contrast, they were highly resistant to ampicillin, then oxacillin, amoxicillin, tetracycline, cefotaxime, and nalidixic acid. The 16s-Pseudomonas (genus) and the 16s-Pseudomonas aeruginosa (species-specific) genes were detected by PCR in all selected isolates. The virulence genes (pslA and oprL) were found in eight and five studied Ps. aeruginosa isolates, respectively. It is being concluded that the main gene responsible for the virulence of studied Ps. aeruginosa isolated from freshwater fishes is pslA. So, this study also sheds light on the public health concerns of Pseudomonas for consumers. | ||||
| Keywords | ||||
| Pseudomonas aeruginosa; bacteriological studies; molecular studies; African sharptooth catfish; Nile tilapia | ||||
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