MICROBIOLOGICAL AND HISTOPATHOLOGICAL INSIGHTS ON CANINE AND FELINE PYOMETRA | ||||
| Assiut Veterinary Medical Journal | ||||
| Article 38, Volume 71, Issue 186, July 2025, Page 465-476 PDF (830.7 K) | ||||
| Document Type: Research article | ||||
| DOI: 10.21608/avmj.2025.364383.1607 | ||||
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| Authors | ||||
M.M. ELMANSY   1; WAFAA RAGAB 2; H. EL-SHEIKH ALI1; A.M. MAHMOUD1
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| 1Department of Theriogenology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt | ||||
| 2Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt. | ||||
| Abstract | ||||
| Pyometra is a life-threatening condition affecting both bitches and queens, which should be diagnosed and treated early to save the animal's life. This study aimed to identify the recent bacterial isolates associated with pyometra and determine the most effective antibiotic for the treatment according to the sensitivity test. Histopathological examinations have also been performed. The study involved 17 queens and 8 bitches presented to the Teaching Hospital of the Faculty of Veterinary Medicine, Mansoura University, with a mean age of 4.56 years for queens and 4.75 years for bitches. Diagnosis of pyometra depended on history, clinical signs, physical examination, and ultrasonographic imaging. After ovariohysterectomy (OHE), the samples were collected under complete aseptic conditions for bacterial isolation and identification. The study revealed that the most common isolated bacteria from both bitches and queens was E. coli, which represents 37.5% and 47%, respectively. Other bacterial species were isolated, such as Klebsiella, Enterobacter, and Proteus. Some samples showed no growth, which may be attributed to the antimicrobial treatment before the collection of samples, the infection with anaerobic bacteria, or misdiagnosis with mucometra or hydrometra, which are usually not associated with infection. The most sensitive antimicrobial drug, doxycycline (DO), emerged as the most effective antimicrobial, with 83.3% of bacteria being sensitive. Some resistance genes for antibiotics have been detected using PCR, such as blaTEM and blaSHV in E. coli, which were responsible for the resistance to the beta-lactam group, and the qnrS gene encodes resistance to the quinolones. Histologically, there was heavy lymphocytic cell infiltration with cystic endometrial glands and endometrial hemorrhage. In conclusion, early detection and careful monitoring of antimicrobial susceptibility are crucial before therapeutic intervention to decrease the chance of antimicrobial resistance. | ||||
| Keywords | ||||
| : Bitches; queens; Doxycycline; Cystic Endometrial Hyperplasia | ||||
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