A prospective chitosan produced from a biological source: Extraction, Characterization, and Assessment of biological performance | ||||
| Journal of Basic and Environmental Sciences | ||||
| Volume 12, Issue 3, July 2025, Page 133-151 PDF (1.06 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jbes.2025.396559.1023 | ||||
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| Authors | ||||
Ghada E Dawwam  1; Fatma Mohamed2; Fatma El-Shall3; Mohamed EL Awady4; Mohamed Yassin2
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| 1Egypt | ||||
| 2Botany and Microbiology Department, Faculty of Science, Benha University, Benha 13518, Egypt | ||||
| 3Dyeing, Printing and Textile Auxiliary Department, National Research Centre, 33 El-Buhouth St., Dokki, Giza P.O. 12622, Egypt. | ||||
| 4Microbial Biotechnology Department, Biotechnology Research Institute, National Research Centre, 33 El-Buhouth St., Dokki, Giza P.O. 12622, Egypt. | ||||
| Abstract | ||||
| Chitosan, a biodegradable and biocompatible biomaterial, was extracted alongside chitin from Black Soldier Fly Pupae (BSFP). In our study, chitin and chitosan were extracted from Black Soldier Fly (BSF) and assessed for their biomedical applications. Characterization was performed using ATR-FTIR, XRD, and UV–Vis spectroscopy. The chitin content accounted for approximately 36.8% of the pupal dry mass. Extracted chitosan exhibited notable biological activities: moderate antibacterial and antifungal effects against Bacillus subtilis ATCC-6633, Staphylococcus aureus NRRLB-767, Escherichia coli ATCC-25922, Pseudomonas aeruginosa ATCC-10145, Candida albicans ATCC-10231, and Aspergillus niger NRRLA-326, significant antioxidant properties (e.g., 36.98% 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 31.33% 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging at 500 µg/ml), and metal ion chelation (30.83%). It inhibited lipid peroxidation (35.39%) and demonstrated free radical scavenging activity (SOR: 38.28%, NO: 26.40%). Anti-inflammatory assays showed inhibition of COX-1 (50.20%) and COX-2 (49.61%). Additionally, chitosan showed cytotoxicity against several cancer cell lines (Wi-38, HepG2, CaCo-2, and A549) with IC₅₀ values ranging from 19.36 to 41.17 µg/ml. Thus these results support the potential of chitosan as an antimicrobial, anti-inflammatory, and anticancer agent. | ||||
| Keywords | ||||
| Chitin Black Soldier Fly; Antimicrobial activity; Antioxidant activity; cytocompatibility | ||||
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