Biological control of Trichoderma harzianum to Alternaria alternate the existence of growth regulator benzene adenine | ||||
| Journal of Bioscience and Applied Research | ||||
| Articles in Press, Accepted Manuscript, Available Online from 31 July 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jbaar.2025.396574.1231 | ||||
 View on SCiNiTO | ||||
| Author | ||||
ali m saadi 
| ||||
| Department of Anesthesia Techniques, Mosul Medical Technical Institute, Northern Technical University | ||||
| Abstract | ||||
| The effectiveness of Trichoderma harzianum isolates as biocontrol agents against Alternaria alternata, the causative agent of leaf spot disease in faba (Visia faba), was tested by testing the antigenicity of isolates to A.alternata. These isolates varied in their antigenicity, with the Th1 isolate achieving the highest antigenicity, with a Bell score of 2.5. The effectiveness of T. harzianum isolates in producing cellulase, pectin, and protease enzymes was then tested. All isolates demonstrated efficacy in producing these enzymes, with the most efficient isolates being Th1 (18, 20, 27 mm radius of the lytic halo around the fungal colony) and Th4 (15, 17, 22 mm radius of the lytic halo around the colony) for all enzymes, respectively. Then, a test was carried out to add different concentrations of the growth regulator benzene adenine (BA) to the diameters of A. alternata fungal colonies. The growth rate of the pathogenic fungus decreased with increasing concentration of the growth regulator compared to the control isolate, while it did not significantly affect the growth of Th1 fungal colonies. Thus, it was observed that the growth of A. alternata was affected when grown on polygalacturonase medium, while the Th1 fungal isolate was not affected when grown on polygalacturonase medium at different concentrations, to a lesser extent. When conducting an antisense test between A. alternata and T. harzianum on PDA and polygalacturonase medium using different concentrations of BA, the diameter rates of A. alternata decreased, while the diameters of T. harzianum were normal, indicating that the presence of | ||||
| Keywords | ||||
| Trichoderma harzianum; Alternaria alternate; Benzene adenine; Biological control | ||||
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