COMBINING ABILITY AND GENETIC DIVERSITY OF PROMISING MAIZE (Zea mays L.) INBRED LINES UNDER VARYING WATER REGIMES ASSESSED VIA DIALLEL ANALYSIS AND RAPD MARKERS
Mohamed, A., Bakry, H., Soltan, H., Asaad, M. (2025). COMBINING ABILITY AND GENETIC DIVERSITY OF PROMISING MAIZE (Zea mays L.) INBRED LINES UNDER VARYING WATER REGIMES ASSESSED VIA DIALLEL ANALYSIS AND RAPD MARKERS. EKB Journal Management System, 52(3), 513-527. doi: 10.21608/zjar.2025.445122
Ahmed M. El. Mohamed; Hanaa S. Bakry; H. A. H. Soltan; M. R. Asaad. "COMBINING ABILITY AND GENETIC DIVERSITY OF PROMISING MAIZE (Zea mays L.) INBRED LINES UNDER VARYING WATER REGIMES ASSESSED VIA DIALLEL ANALYSIS AND RAPD MARKERS". EKB Journal Management System, 52, 3, 2025, 513-527. doi: 10.21608/zjar.2025.445122
Mohamed, A., Bakry, H., Soltan, H., Asaad, M. (2025). 'COMBINING ABILITY AND GENETIC DIVERSITY OF PROMISING MAIZE (Zea mays L.) INBRED LINES UNDER VARYING WATER REGIMES ASSESSED VIA DIALLEL ANALYSIS AND RAPD MARKERS', EKB Journal Management System, 52(3), pp. 513-527. doi: 10.21608/zjar.2025.445122
Mohamed, A., Bakry, H., Soltan, H., Asaad, M. COMBINING ABILITY AND GENETIC DIVERSITY OF PROMISING MAIZE (Zea mays L.) INBRED LINES UNDER VARYING WATER REGIMES ASSESSED VIA DIALLEL ANALYSIS AND RAPD MARKERS. EKB Journal Management System, 2025; 52(3): 513-527. doi: 10.21608/zjar.2025.445122

COMBINING ABILITY AND GENETIC DIVERSITY OF PROMISING MAIZE (Zea mays L.) INBRED LINES UNDER VARYING WATER REGIMES ASSESSED VIA DIALLEL ANALYSIS AND RAPD MARKERS

Zagazig Journal of Agricultural Research
Volume 52, Issue 3, May and June 2025, Page 513-527  XML PDF (1.16 MB)
Document Type: Original Article
DOI: 10.21608/zjar.2025.445122
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Authors
Ahmed M. El. Mohamed email orcid 1; Hanaa S. Bakry2; H. A. H. Soltan3; M. R. Asaad4
1. Agron. Dept., Fac. Agric., Minia Univ., El-Minia, 61519, Egyptt.
2Genet. Dept., Fac. Agric., Minia Univ., 61519 Minia, Egypt
3Cent. Lab. of Organic Agric., Agric. Res. Cent. (ARC), 12619 Giza, Egypt
4Agron. Dept., Fac. Agric., Minia Univ., El-Minia, 61519, Egypt
Abstract
: Six white maize inbred lines were used to produce 15 F1 crosses, which were evaluated under normal and water-stressed conditions. To determine the combining ability, 21 maize genotypes (parental lines and their respective hybrids) were evaluated during 2024 season. The genotypes exhibited highly significant mean squares for all studied traits under both watering regimes, except for anthesis-silking interval (ASI), number of rows (No.R) (under stress). The parental vs. crosses (P.vs.C.) contrast showed significant differences for all studied traits under both conditions, except, ASI and shelling percentage (SH%) under both conditions. P1 recorded significant negative general combining ability (GCA) effects for ear weight (EW) under both conditions. However, P3, P4 and P6 (under normal conditions) showed significant positive GCA effects. All crosses showed significant positive specific combining ability (SCA) effects for EW and kernel weight (KW) under both conditions, reflecting the importance of its parental combinations in enhancing productivity. Based on the molecular analysis results, it can be concluded that all six RAPD primers used were successful in verifying identity the studied maize genotypes, particularly the four primers (OPL-01, OPK-10, OPM-04 and OPM-05), which generated 6, 5 and 3 unique bands, respectively. These markers can be utilized to distinguish among genotypes. The highest similarity value (0.977) was observed between the two maize hybrids H6 and H12, while the lowest one was (0.414) observed between the two parents P4 and P6. The molecular findings based on RAPD marker analysis revealed clear genetic diversity among the maize parents and their hybrids, which is crucial for guiding the selection of parental lines in hybridization programs to enhance heterosis and maintain broad genetic variability in maize improvement efforts.
Keywords
Maize; Diallel; Combining ability; Genetic diversity; RAPD marker
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