Total Phenolic and Flavonoid Contents, Antibacterial Evaluation, and Bioactive Compounds Identification in Calliandra surinamensis Benth. cultivated in Egypt

Zeid, Tasneem Zedan; Bayoumi, Soad A. L.; Mamdoh, Hend; Ahmed, Amany S.; Ramadan, Mahmoud A.

doi:10.21608/bfsa.2025.403719.2641

	Total Phenolic and Flavonoid Contents, Antibacterial Evaluation, and Bioactive Compounds Identification in Calliandra surinamensis Benth. cultivated in Egypt
Bulletin of Pharmaceutical Sciences Assiut University
Articles in Press, Accepted Manuscript, Available Online from 03 August 2025
Document Type: Original Article
DOI: 10.21608/bfsa.2025.403719.2641
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Authors
Tasneem Zedan Zeid ¹; Soad A. L. Bayoumi²; Hend Mamdoh³; Amany S. Ahmed²^{, 1}; Mahmoud A. Ramadan²
¹Department of Pharmacognosy, Faculty of Pharmacy, Sphinx University, Assiut, Egypt
²Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
³Department of Microbiology and Immunology, Faculty of Pharmacy, Sphinx University, New Assiut, Egypt.
Abstract
Calliandra surinamensis is an ornamental plant widely distributed across tropical and subtropical regions, and it is cultivated in Egypt. Despite its traditional use, there have been limited studies exploring its phytochemical composition and biological activities. This study aimed to evaluate the total phenolic and flavonoid contents of the plant crude methanolic extract along with phenolic-rich fractions, to isolate phytoconstituents from the most active fraction using different chromatographic techniques, to identify their structures using 1H and 13C NMR techniques, and to assess the antibacterial activity of the extract and all subsequent fractions. The ethyl acetate fraction exhibited the highest total phenolic and flavonoid content. Phytochemical investigation of this fraction led to the isolation of four phenolic compounds: methyl gallate, kaempferol-3-O-rhamnoside (afzelin), quercetin-3-O-rhamnoside (quercitrin), and myricetin-3-O-rhamnoside (myricitrin). Interestingly, the ethyl acetate fraction showed the strongest antibacterial activity against Escherichia coli bacterial strains. These findings suggest that C. surinamensis may serve as a promising source of natural antioxidants and antibacterial agents. Further in vivo studies are recommended to confirm its safety and therapeutic potential.
Keywords
Calliandra surinamensis; Fabaceae; Flavonoids; Chromatography; Antibacterial activity


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