Molecular Characterization, Serotyping, and Antimicrobial Resistance Profiles of Escherichia coli Isolates from Companion Animals in El-Menoufia Governorate, Egypt | ||||
| Menoufia Veterinary Medical Journal | ||||
| Volume 1, Issue 1, August 2025, Page 1-18 PDF (1.14 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmmj.2024.332948.1020 | ||||
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| Author | ||||
Rawda Ibraheim Essa 
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| Bacteriology, immunology and mycology, veterinary medicine menofia university,Shebin elkom, El menofia | ||||
| Abstract | ||||
| All domestic animals and the environment contain Escherichia. coli, which is easily dispersed throughout various compartments. E. Coli is also a "highly relevant and representative indicator of the global antimicrobial resistance (AMR) problem," according to the World Health Organization (WHO). It is categorized into multiple pathotypes that cause intestinal and extraintestinal diseases, including skin and soft tissue infections, gastroenteritis, urinary tract infections (UTI), and septicemia. A bacteriological analysis of 100 pet animal samples from the El-Menoufia governorate (40 dogs, 50 cats, and 10 Egyptian nisnas) showed that isolation from dogs (47.5%) ,cats (42%) and Egyptian nisnas (20%). Isolates of E.coli were tested by PCR. The 16SrRNA gene was found in all isolates at 401 pb. E.coli is a worldwide source of antibiotic resistance. We looked at using multiplex PCR to find the genes that are resistant to beta lactamases (blaSHV, blaTEM, blaCTXM, and blaOXA). Each isolate has the gene blaTEM, one isolate has the genes blaSHV and blaTEM, and four isolates have the genes blaTEM and blaCTXM. They have been further divided into seven E. coli pathotypes based on the various virulence characteristics and clinical changes brought about by pathogenic E. coli strains: Shiga-like toxin producing (STEC), enterotoxigenic/heat-labile/heat-stable enterotoxins generating (ETEC), diffusely adherent (DAEC), enteroinvasive (EIEC), enterohemorrhagic (EHEC), enteropathogenic (EPEC), and enteroaggregative (EAEC). From pets, phylogroup B2 was found to be quite prevalent. With the exception of two isolates that lack the tspE4.C2 gene, all isolates had the phylogenetic genes chuA, yjaA, and tspE4.C2 detected by PCR. | ||||
| Keywords | ||||
| Antimicrobial resistance; E-coli; pet animals and Phylogroups • | ||||
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