Anti-Sporulation and Morphological Alterations in Eimeria stiedae Oocysts Induced by Acacia Nilotica Extract: Dose-Dependent Efficacy and Phytochemical Insights
Mashhour, A., Abdel-Raheem, M., Morad, S., Abdelmageed Mohamed Fouad, N. (2025). Anti-Sporulation and Morphological Alterations in Eimeria stiedae Oocysts Induced by Acacia Nilotica Extract: Dose-Dependent Efficacy and Phytochemical Insights. EKB Journal Management System, 3(2), 15-24. doi: 10.21608/ijcvr.2025.338640.1009
Amany Mashhour; Mahmoud Abdel-Raheem; Samy Morad; Noha Abdelmageed Mohamed Fouad. "Anti-Sporulation and Morphological Alterations in Eimeria stiedae Oocysts Induced by Acacia Nilotica Extract: Dose-Dependent Efficacy and Phytochemical Insights". EKB Journal Management System, 3, 2, 2025, 15-24. doi: 10.21608/ijcvr.2025.338640.1009
Mashhour, A., Abdel-Raheem, M., Morad, S., Abdelmageed Mohamed Fouad, N. (2025). 'Anti-Sporulation and Morphological Alterations in Eimeria stiedae Oocysts Induced by Acacia Nilotica Extract: Dose-Dependent Efficacy and Phytochemical Insights', EKB Journal Management System, 3(2), pp. 15-24. doi: 10.21608/ijcvr.2025.338640.1009
Mashhour, A., Abdel-Raheem, M., Morad, S., Abdelmageed Mohamed Fouad, N. Anti-Sporulation and Morphological Alterations in Eimeria stiedae Oocysts Induced by Acacia Nilotica Extract: Dose-Dependent Efficacy and Phytochemical Insights. EKB Journal Management System, 2025; 3(2): 15-24. doi: 10.21608/ijcvr.2025.338640.1009

Anti-Sporulation and Morphological Alterations in Eimeria stiedae Oocysts Induced by Acacia Nilotica Extract: Dose-Dependent Efficacy and Phytochemical Insights

International Journal of Comprehensive Veterinary Research
Volume 3, Issue 2, December 2025, Page 15-24  XML PDF (692.27 K)
Document Type: Research article
DOI: 10.21608/ijcvr.2025.338640.1009
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Authors
Amany Mashhour1; Mahmoud Abdel-Raheem2; Samy Moradorcid 3; Noha Abdelmageed Mohamed Fouad email orcid 4
1Department of Pharmacology, Faculty of Veterinary Medicine, Sohag University, Sohag, Egypt
2Medical Pharmacology Department, Faculty of Medicine, Assiut University, Assiut, Egypt
3Department of Pharmacology, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt
4Ass. Professor of Pharmacology, Faculty of Veterinary Medicine - Sohag University
Abstract
This study explorer the inhibitory effects of Acacia nilotica pod extract (ANAPE) on sporulation and coccidial score reduction of Eimeria stiedae oocysts, Main cause of Hepatic coccidiosis in Rabbits colonies. The results demonstrate a dose-dependent reduction in sporulation index with increasing ANAPE concentrations, with a half-maximal inhibitory concentration (IC50) of 58 mg/ml. A dose-response curve exhibits a linear relationship between ANAPE concentration and inhibition percentage. Additionally, ANAPE treatment significantly reduces coccidial scores in a dose-dependent manner, with the highest concentration (100 mg/ml) showing the most potent effects. The study also reveals dose-dependent morphological (size and shape) alterations in Eimeria oocysts induced by ANAPE exposure, characterized by increases in overall size and alterations in shape. Phytochemical analysis of ANAPE indicates the presence of various secondary metabolites, including carbohydrates, flavonoids, glycosides, saponins, and tannins. These findings suggest that ANAPE possesses potent anti-sporulation properties and may be a valuable agent in controlling Eimeria oocyst sporulation.
Keywords
Acacia nilotica; Eimeria stiedae; sporulation; coccidial score; phytochemical profile
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