Diversity studies by using RAPD-PCR and antibiogram discrimination of klebsiella species isolated from pets and co-habitant human | ||||
| Menoufia Veterinary Medical Journal | ||||
| Volume 1, Issue 1, August 2025, Page 1-13 PDF (949.99 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/vmmj.2024.319712.1016 | ||||
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| Author | ||||
Rawda Khaled Mashaly 
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| Faculty of Veterinary Medicine Department of Bacteriology, Mycology and Immunology | ||||
| Abstract | ||||
| Klebsiella is readily transmissible among various species, encompassing both humans and animals. It plays a crucial role in the occurrence of nosocomial infections, including pneumonia, sepsis, and urinary tract infections. The aim of this research was to ascertain the genetic similarities between Klebsiella isolates obtained from pet animals and humans through the application of Random Amplified Polymorphic DNA (RAPD-PCR) technology. Traditional microbiological methods were employed to assess the prevalence of Klebsiella spp. in both pet animals and their human cohabitants. The specimens were obtained from pet animals specifically cats and dogs, through nasal swabs, fecal samples and urine samples (n = 100), while human samples were collected from patients that had infection in respiratory and urinary tracts from labs and cohabitating humans with pet animals in houses (n = 50) (sputum and urine samples) at El-Menoufia and El-Garbia Governorates. Conventional techniques identified 26 out of 150 isolates of Klebsiella spp. from pet animals and human, 7/50 (14%) from dogs and 11/50 (22%) from cats) and 8/50 (16%) from human samples. The incidence of K.pneumoniae was 16/150 (10.6%), and K.oxytoca was 10/150 (6.66%). Antimicrobial susceptibility test revealed that Klebsiella isolates from pets and human showing 100% completely resistant to amoxicillin\calvulante and 80% sensitive to ciprofloxacin for Klebsiella isolates from pets. The Polymerase Chain Reaction (PCR) and Random Amplified Polymorphic DNA (RAPD) PCR techniques were utilized to validate the Klebsiella isolates and ascertain the genetic fingerprints among them. | ||||
| Keywords | ||||
| Klebsiella species; pets; human and (RAPD) PCR | ||||
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