Comparative Histological Study on the Therapeutic Role of Bone Marrow derived Mesenchymal Stem Cells versus their Derived Exosomes on Experimentally Induced Liver Fibrosis in Adult Male Albino Rat | ||||
| Egyptian Journal of Histology | ||||
| Article 4, Volume 48, Issue 2, June 2025, Page 445-458 PDF (1.66 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2025.351566.2194 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Alaa Mostafa Kassem1; Sara Abd El Gawad Elsebay1; Mohamed Abd El Rahman Ahmed Mekawy 2; Heba Fawzy  1
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| 1histology,faculty of medicine,ain shams university | ||||
| 2Histology Faculty of Medicine Ain Shams University | ||||
| Abstract | ||||
| Background: Liver fibrosis is a significant global health issue with limited treatment options, except for the elimination of the underlying cause or liver transplantation. Both bone marrow mesenchymal stem cells (BM-MSCs) and their exosomes (BM-MSC-EXOs) have demonstrated promise as therapeutic agents due to their ability to regenerate and immunomodulate. Aim of the Work: To compare the therapeutic role of BM-MSCs and their exosomes on the liver's structure in adult male albino rats with carbon tetrachloride-induced liver fibrosis. Material & Methods: Forty-five adult male albino rats were included in this study, 5 of them used to isolate MSCs. Group I (the control group) included ten rats. Group II included thirty rats that received CCl4 to induce liver fibrosis then subdivided equally into subgroup IIA (CCl4 group), subgroup IIB (BM-MSCs group) received BM-MSCs once and subgroup IIC (EXOs group) received EXOs once. Results: CCl4 injection significantly induced hepatic dysfunction. It caused marked histological alternations, as confirmed by H&E, electron microscopy examination, and biochemical analysis of liver function. Rats treated with BM-MSCs, and exosomes showed considerable improvement in comparison with the CCl4-treated group. There was also a significant decrease in both the area percentages of collagen and TNF-α positive hepatocytes in these groups compared to CCl4-treated group. Although the rats treated by exosomes showed more improvement in all H&E, ultrastructure, and biochemical analysis, statistically there was a non-significant difference between BM-MSCs and exosome groups. Conclusion: The study revealed that the therapeutic role of BM-MSCs-EXOs seemed to have a greater effect than the parent BM-MSCs in a rat model of liver fibrosis induced by CCl4. | ||||
| Keywords | ||||
| BM-MSCs; CCL4; exosomes; liver fibrosis; TNF-α | ||||
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