Biosynthesis of gold nanoparticles using local Pseudomonas aeruginosa isolates and their synergistic effects against multidrug-resistant pathogenic bacteria | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 05 August 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2025.370551.2646 | ||||
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| Authors | ||||
Suaad A. F. Al-miyah 1; Hanan Saad Hashim 2; Alaa Hashim Abd Ali  2, 3; Hadeel T.A. Al-Najem 2
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| 1Department of Biology, College of Sciences, University of AL-Qadisiyah, Iraq | ||||
| 2Department of Medical Laboratory Techniques, College of Health and Medical Techniques, Al-Furat Al-Awsat Technical University, Kufa, Iraq | ||||
| 3Academy of Biology and Biotechnology,Southern Federal University, 344006, st. B. Sadovaya, 105/42, Rostov-on-Don, Russia | ||||
| Abstract | ||||
| Background: Due to their reputation as ecologically friendly bacteria and their contribution to the advancement of nanotechnology, Biological sources, such as microorganisms, significantly facilitate the reduction of metallic nanoparticles like gold. Objectives: In this experiment, Gold nanoparticles are biologically synthesized using the culture supernatant of local Pseudomonas aeruginosa isolates. Methods: Individually, 1 × 10⁻³ M gold chloride trihydrate (HAuCl₄) was added to the supernatant. The production of gold nanoparticles and their surface morphology was examined using Atomic Force Microscopy (AFM). In addition, the stability and production of gold nanoparticles in water solutions were determined, and the presence of different groups was verified using UV-visible and Fourier Transform Infrared (FTIR) spectroscopy, respectively. The antimicrobial effectiveness of metal nanoparticles against Multidrug-Resistant (MDR) bacteria has been assessed both individually and in conjunction with drugs that are accessible and commercially obtainable. Results: The results show that the antibacterial activity of Ciprofloxacin, azithromycin, cefoxitin, and clindamycin increases in the presence of AuNPs against test strains. The enhanced synergistic effect may be attributed to the interaction between the antibiotic and gold nanoparticles. The complementary advantages of using NPs/nanotechnologies as antibacterial agents compared with traditional antibiotics is overcoming the existing antibiotic resistance mechanisms including the reduced and disruption of bacterial membranes. Conclusion: The synergistic effects of biosynthesizing AuNPs with antibiotics at different concentrations against MDR bacteria are more effective than native antibiotics against MDR bacteria. Therefore, gold nanoparticles are an effective material for drug delivery and can potentially address antimicrobial resistance challenges. | ||||
| Keywords | ||||
| Pseudomonas aeruginosa; biosynthesis; gold nanoparticles; antimicrobial synergy; surface plasmon resonance | ||||
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