Role of Heat Shock Pretreated Bone Marrow Mesenchymal Stem Cells Versus Non-Treated Ones in Repair of Experimentally Induced Premature Ovarian Insufficiency by Doxorubicin | ||||
| Egyptian Journal of Histology | ||||
| Article 18, Volume 48, Issue 2, June 2025, Page 657-672 PDF (1.62 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2024.269354.2019 | ||||
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| Authors | ||||
Rawda Fouad Mostafa  1; Makram Fahmy Sidhom2; Mona mohammed Zoair1; Manal Ibrahim El-Bermawy1; kadreya elsayed elmorshedy 3
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| 1Anatomy and embryology. Faculty of medicine. Tanta University | ||||
| 2Anatomy and embryology faculty of medicine tanta university | ||||
| 3anatomy and embryology tanta college of medicine.egypt and king khalid university saudia arabia | ||||
| Abstract | ||||
| Introduction: Premature ovarian insufficiency (POI) is one of the most severe problems threatening the reproductive health of women of normal childbearing age. Aim of the Work: This study aimed to throw a light on the effect of heat shock (HS) pretreated bone marrow mesenchymal stem cells (BM- MSCs) versus non-treated ones in repair of DOX-induced POI. Material and Methods: Fifty-six adult female albino rats were utilized and divided into three groups; Stem cells donor group included 10 rats, Control Group included 16 rats, Experimental group (30 rats); each rat received (1ml) 2.1 mg/kg BW Doxorubicin (DOX) once every 3rd day for seven IP injections. Then, this group was subdivided into three subgroups (n=10 each); subgroup III-A (DOX- treated), subgroup III-B (DOX+ routine BM-MSCs) and subgroup III-C (DOX+ HS-pre-treated BM-MSCs). Green fluorescent protein (GFP) was used for labeling and tracing the injected BM- MSCs. E2 and FSH levels were measured, rats were sacrificed and ovarian specimens were possessed for histological, immuno-histochemical, morphometric studies and statistical analysis. Results: The ovarian function in rats that received heat shock pre-treated BM-MSCs revealed significant increase of the serum level of E2 and significant decrease of FSH than in rats received routine ones. Heat shock pre-treatment increased homing of BM-MSCs which was proved by the significant increase of fluorescence traced GFP-labeled BM-MSCs in subgroup III-C as compared to subgroup III-B. There was marked improvement in the histological structure of ovarian sections of the rats received heat shock pre-treated BM-MSCs more than those received routine ones with significant decrease of apoptosis evidenced by caspase-3 immunoreactivity. Conclusion: Heat shock pre-treated BM-MSCs had a great effect in reducing the degenerative changes associated with DOX induced POI than the routine ones. | ||||
| Keywords | ||||
| BM-MSCs; doxorubicin; GFP; heat shock; POI | ||||
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