Evaluation of the efficacy and safety of DAMPI, a traditional herbal recipe, used for treatment of male infertility | ||||
| Egyptian Journal of Histology | ||||
| Articles in Press, Accepted Manuscript, Available Online from 07 August 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2025.355039.2271 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Simon AZONBAKIN  1; Marius ADJAGBA2; Gerard DOSSOU GOUIN3; Mariano AMOUSSOU4; Paul KOURAOGO5; Habib GANFON6; Godwin SOGLO5; Anatole LALEYE5; Bonaventure AWEDE7
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| 101 BP Campus Universitaire du Champ de Foire Cotonou | ||||
| 2Unité de Biologie Humaine, Faculté des Sciences de la Santé-Université d'Abomey-Calavi | ||||
| 32Laboratory of Pharmacognosy and Phytotherapy at the Faculty of Health Sciences of Cotonou, University of Abomey-Calavi, Benin | ||||
| 41Laboratory of Histology Biology of Cytogenetics Reproduction and Medical Genetics at the Faculty of Health Sciences of Cotonou, University of Abomey-Calavi, Benin | ||||
| 5Laboratory of Histology Biology of Cytogenetics Reproduction and Medical Genetics at the Faculty of Health Sciences of Cotonou, University of Abomey-Calavi, Benin | ||||
| 6Laboratory of Pharmacognosy and Phytotherapy at the Faculty of Health Sciences of Cotonou, University of Abomey-Calavi, Benin | ||||
| 7Unity of Physiology, Faculty of Health Sciences of Cotonou, University of Abomey-Calavi, Benin | ||||
| Abstract | ||||
| Introduction: Since several centuries, medicinal plants have been used to heal human diseases such as infertility in many African countries. Objective: This study aimed to assess the efficacy and safety of DAMPI, a traditional recipe made from an association of Desmodium velutinum, Aframomum melegueta, Musa paradisiaca, Piper guineense, and Vitellaria paradoxa used in the treatment of male infertility in male infertility wistar rat model. Methods: Phytochemical analysis was done through Wagner and Bladt method. The total phenolics, flavonoid and flavanol content of the recipe were respectively estimated via Folin-ciocalteu, Aluminium chloride and “ Vanillin chloride” methods. The protective effect of DAMPI against hydrogen peroxide (H₂O₂) induced seminal quality deterioration was assessed on male Wistar rat model. Twenty-five (25) rats were divided into five groups of five rats each. Negative control group received distilled water while positive control(H₂O₂ group) received H₂O₂ at 0.5% . DAMPI groups received 0.5% H₂O₂ and DAMPI at respectively 0.5 g/kg and 1 g/kg. The reference Veliten group received H₂O₂ at 0.5% and Veliten (Tocopherol acetate (50 mg/kg) + ascorbic acid (200 mg/kg) + Rutin (200 mg/kg)). The treatment was administered for 60 days through gavage. preliminary safety of DAMPI test was performed according to OECD guidelines 423 on two groups of three rats each via gavage. The control group received DMSO while the treatment group received DAMPI at 2000 mg/kg. Results: Phytochemical analysis of DAMPI revealed the presence of flavanol , flavonoids, alkaloids, leucoanthocyanins, reducing sugar, steroids and triterpenes. The H₂O₂ induced a marked alteration on seminal picture, impaired the seminiferous tubules organization, destructed the seminiferous epithelium, increased MDA (malondialdehyde) levels and decreased antioxidant enzyme activity. Conclusion: This study demonstrated the efficacy of DAMPI against oxidative stress induced alterations on semen parameters and it also exhibits the lack of acute toxicity of DAMPI. | ||||
| Keywords | ||||
| DAMPI; male infertility; oxidative stress; traditional herbal recipe; Wistar rat | ||||
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