Phytochemical Profiling, Cytotoxicity and Effect of Ethanol and Aqueous Fractions of Celtis Integrifolia against Monosodium Glutamate-Induced Neurodegenerative Diseases Like Behavioral Symptoms in Mice | ||||
| African Journal of Biological Sciences | ||||
| Articles in Press, Accepted Manuscript, Available Online from 07 August 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ajbs.2025.372534.1125 | ||||
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| Authors | ||||
Bih Belta Lilian Fubi1; Kada Sanda Antoine2; Tangu Patience Neng2; Mumbi Laurantine Nguenteh2; Ndifor Rose Nchang2; Oumar Mahamat   3
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| 1Department of Zoology, Faculty of Sciences, Univeristy of Bamenda | ||||
| 2Department of Zoology, Faculty of Science, The University of Bamenda, Cameroon | ||||
| 3Department of Zoology, Faculty of Sciences, University of Bamenda, Cameoon | ||||
| Abstract | ||||
| Background: Celtis integrifolia is commonly found in Northern parts of Cameroon. The roots are used in traditional medicine to alleviate nervous problems. The current study sought to assess the neuroprotective potential of C. integrifolia against monosodium glutamate (MSG)-induced neurodegenerative like-behaviour in mice. Methods: Aqueous and ethanol fractions of C. integrifolia were investigated for its phytochemicals and cytotoxicity studies (MTT and Hemolysis tests). In different groups of mice (N=5), the extracts were also studied against the effect of MSG in mice. The behavioural paradigms (Y-maze, Open field test (OFT) and Beam walking test) were performed to evaluate spatial and working memory, exploratory and motor coordination. Results: Findings obtained revealed that different doses of aqueous and ethanol extract fractions of C. integrifolia significantly reversed the adverse effect of MSG on memory. This was evidence by a significant (p<0.001) increment in the percentage of spontaneous alternation in the Y-maze test and a significant (p<0.001) increase in the total distance and number of lines crossed in OFT. IC50 of the ethanol and water fractions were equal to 510.01 mg/ml and 436.78 mg/ml in hemolytic test. Using the MTT test the IC50 were 241.85 mg/ml 211.44 mg/ml in respectively for the ethanol and water fractions. Both water and ethanol fractions of C. integrifolia are rich of flavonoids, saponins and alkaloids. Conclusion: The present study demonstrated the root of C. integrifolia is not toxic and has a potential neuroprotective that can investigate for treatment of various neurodegenerative diseases. | ||||
| Keywords | ||||
| Celtis integrifolia; Cognitive impairment; Cytotoxicity; Monosodium glutamate | ||||
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