Study of MicroRNA-146a Expression and Soluble Triggering Receptor Expressed on Myeloid Cells-1(sTREM-1) Serum Levels in Patients with Sepsis | ||
| Egyptian Journal of Medical Microbiology | ||
| Volume 35, Issue 2, April 2026 | ||
| Document Type: New and original researches in the field of Microbiology. | ||
| DOI: 10.21608/ejmm.2025.410674.1823 | ||
| Authors | ||
| Reem R. Abd El-Glil1; Gamal A. Amer1; Engy A. Okab2; Marwa A. Elaraby* 1; Hasnaa S. Abd El-Hamid1 | ||
| 1Department of Medical Microbiology and Immunology, Faculty of Medicine, Benha University, Egypt | ||
| 2Department of Intensive Care Unit (ICU), Faculty of Medicine, Benha University, Egypt | ||
| Abstract | ||
| Background: Sepsis represents a critical condition characterized by organ dysfunction resulting from exaggerated immune response to infection. MiRNA-146a is vital mediators in innate immune and inflammatory responses and its association with pro-inflammatory cytokines production, lipopolysaccharide (LPS)-mediated. TREM-1 is defined as an innate immunity receptor expressed on myeloid cells as monocytes and neutrophils involved in their activation and release of cytokine. Objectives: To study microRNA-146a expression and sTREM-1 serum levels in patients with sepsis compered to control healthy group and assess their diagnostic and prognostic value. Methodology: This study was carried out on 90 participants: 60 septic patients from ICU Department, Benha University Hospital on the basis of blood culture (positive microbiological culture results) and National Early Warning Score (NEWS), 30 age and sex matched healthy controls. Five ml of venous blood was collected in sterile test tube with no anticoagulants from all participants under complete aseptic condition. Serum was separated and divided in to 2 parts for Real time PCR for measurement miRNA 146-a expression and assess sTREM-1 levels by ELISA. Results: miRNA 146a gene expression increased significantly in the sepsis group compared to the control group (p<0.001) with median of 3.05x10⁴. sTREM-1 serum levels were also significantly higher in sepsis patients with a median level of 347.28 (p<0.001) against 104.12 in the control group. Conclusion: miRNA-146a and sTREM-1 have excellent diagnostic value in sepsis. They have potential utility in monitoring disease prognosis for better clinical outcomes. | ||
| Keywords | ||
| Sepsis; miRNA146a; RT-PCR; sTREM-1; ELISA | ||
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