Does GC Content of Random Amplified Polymorphic DNA (RAPD) Markers Influence Their Discrimination of Different Biological Specimens? | ||
| Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology | ||
| Volume 17, Issue 2, December 2025, Pages 109-136 PDF (1.02 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/eajbsc.2025.446808 | ||
| Authors | ||
| Thomas, B. T.1; Coker, M. O.1; Thomas, A. .N.2; Popoola, O. .D.1 | ||
| 1Department of Microbiology, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria. | ||
| 2Department of Animal production, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria. | ||
| Abstract | ||
| A set of 2000 RAPD works involving different biological specimens was compiled and analyzed for the presence of discrete amplification to deduce the impacts of the percentage of nitrogenous bases (Guanine-Cytosine content) of RAPD markers on their discrimination of various biological specimens. Articles lacking data on RAPD amplification were excluded, while only 56 articles having a total of 761 decamer primers were subsequently subjected to logistic statistical analysis (Analysis of variance and correlation analysis). Results obtained revealed GC contents between 60-70%, 60-70%, 65%, 55% and 55-65% as the best for RAPD discrimination of bacteria, plants, insects, protozoans, and fungi, respectively. The bivariate correlation analysis also documents a significant relationship between GC contents of RAPD markers and their levels of polymorphisms. Our results, however, emphasize that the challenge faced with large-scale RAPD-PCR through several optimization techniques may be averted with judicious use of GC content as a predictor of the level of polymorphisms. | ||
| Keywords | ||
| Guanine-Cytosine content; RAPD; Bacteria; Fungi; Insects; Protozoans; plants | ||
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