Optimizing the conditions of phycocyanin extraction and purification from Spirulina sp.
El hady Belal, S., Soliman, H., Refaay, D., Hussein, M., Mowfy, A. (2024). Optimizing the conditions of phycocyanin extraction and purification from Spirulina sp.. EKB Journal Management System, 71(4), 44-50. doi: 10.21608/mjb.2024.447300
Shaimaa A. El hady Belal; Hoda M. Soliman; Dina A. Refaay; Mervat H. Hussein; Amr M. Mowfy. "Optimizing the conditions of phycocyanin extraction and purification from Spirulina sp.". EKB Journal Management System, 71, 4, 2024, 44-50. doi: 10.21608/mjb.2024.447300
El hady Belal, S., Soliman, H., Refaay, D., Hussein, M., Mowfy, A. (2024). 'Optimizing the conditions of phycocyanin extraction and purification from Spirulina sp.', EKB Journal Management System, 71(4), pp. 44-50. doi: 10.21608/mjb.2024.447300
El hady Belal, S., Soliman, H., Refaay, D., Hussein, M., Mowfy, A. Optimizing the conditions of phycocyanin extraction and purification from Spirulina sp.. EKB Journal Management System, 2024; 71(4): 44-50. doi: 10.21608/mjb.2024.447300

Optimizing the conditions of phycocyanin extraction and purification from Spirulina sp.

Mansoura Journal of Biology
Volume 71, Issue 4, December 2024, Page 44-50  XML PDF (1.45 MB)
Document Type: Original Article
DOI: 10.21608/mjb.2024.447300
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Authors
Shaimaa A. El hady Belal* 1; Hoda M. Soliman1; Dina A. Refaay2; Mervat H. Hussein2; Amr M. Mowfy1
1Botany Department Faculty of Science – Mansoura University
2Botany Department Faculty of Science – Mansoura University.
Abstract
Phycocyanin (C-Pc) is a natural pigment with extensive use in the food, cosmetic, and pharmaceutical industries. Spirulina sp. is regarded as a natural source of C-Pc. The optimal growth conditions increased the pigment content. The extraction and purification process are crucial steps that shouldn't be disregarded to keep phycocyanin's quality at maximum level. Several buffers and concentrations of (NH4)2SO4 were evaluated in this work to extract C-Pc. The optimal buffer was 100 mM KPB at pH 7 with a purity value of 0.809 and C-Pc concentration of 4.32 whereas, the optimal (NH4)2SO4 concentration was 65% with purity ratio 0.91 and the C-Pc concentrations was 4.47. Ion exchange chromatography was used to purify C-Pc, yielding a purity of 1.73 using 50 mM KPB PH 7.
Keywords
Phycocyanin; Spirulina sp; Potassium phosphate buffer; Ammonium sulfate; Pigment
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